Trypanosomes  Occurring  in  Naturally  Infected  Dogs 
A.  The  ‘  Chunga’  Trypanosome. 
Origin.  This  strain  was  derived  from  an  Airedale  bitch  which 
was  brought  North  from  Cape  Colony  at  the  end  of  May.  If  left 
the  railway  on  July  yth.  On  the  5th  August  the  owner,  who  was 
travelling  with  her,  noticed  she  was  getting  rather  weak,  and  that  her 
milk  was  diminishing— -this  was  ascribed  to  the  litter  of  pups  which 
had  been  born  on  July  i8th — but  she  appeared  to  regain  her  condition. 
On  August  27th  an  opacity  of  the  right  cornea  was  noticed,  and 
examination  on  September  4th  at  Chunga  Farm  showed  trypano¬ 
somes. 
Tsetse  flies  {Gl.  morsitans)  might  have  been  encountered  within 
a  few  days  of  the  railway.  They  appear  to  be  constantly  present  in  a 
district  (Mpika)  entered  on  August  ist;  and  it  would  seem  possible 
for  infection  to  have  taken  place  then. 
We  were  informed  that  this  bitch  was  destroyed  in  extremis  on 
September  nth,  a  week  after  diagnosis. 
A  healthy  native  dog  w'as  inoculated  from  this  bitch  and  was 
carried  to  Kambole,  serving  as  the  origin  for  the  experimental  work 
possible  ;  it  was,  however,  rather  our  desire  to  maintain  the  strain  for 
future  investigation  under  suitable  conditions. 
The  following  is  the  genealogy  of  the  strain :  — 
Chunga  bitch 
Dog  I 
Rat  I  Guinea-pig 
4 
Sheep 
2 
Goat 
I 
Ox 
I 
Ox  Guinea-pig 
Dog  Grey  rat 
Goat 
Dog 
Dog  Sheep 
3  12 
II  3 
4 
6 
'3  7 
Dog  17 
Rat  10  (arrived  at 
Pretoria) 
Experimental 
infection 
Rat  I. — This 
animal  had  been  inoculated 
on  May  9th  with  ‘  Scotsdale  ’  strain 
without  result. 
