359 
September  24th.  Received  intraperitoneally  three  drops  blood  of  Dog  i. 
Irypanosomes  appeared  on  the  fifth  day  and  were  constantly  present  till  death 
on  November  gth  (46th  day).  Ten  days  before  death  there  were  incoordination  of 
the  hind  legs,  and  a  muco-purulent  discharge  from  the  conjunctivae. 
Rat  10.  Was  inoculated  subcutaneously  with  o'5  c.cm.  heart  blood  of  Dog 
17  just  dead,  on  December  31st.  No  further  examinations  were  made  till  the 
animal  arrived  in  Pretoria,  when  organisms  were  very  numerous.  It  was  still 
alive  on  January  23rd. 
Grey  Rat.  3.  Caught  locally.  Inoculated  subcutaneously  November  gth,  igo8, 
with  o'5  c.cm.  blood  of  Rat  i.  Trypanosomes  appeared  on  the  fourth  day  and 
were  constantly  seen  up  to  death  on  December  14th,  the  35th  day. 
Guinea-pig  12.  This  animal  had  been  unsuccessfully  inoculated  with 
‘  Scotsdale  ’  strain  in  May,  igo8. 
November  3rd,  inoculated  with  eight  drops  blood  Rat  i,  and  on  this  rat’s 
death,  November  gth,  received  the  heart  washings.  Trypanosomes  were  seen  on 
November  13th,  ten  days  after  first  inoculation.  They  were  present  later  at 
various  times  up  to  death,  which  took  place  on  December  23rd,  and  was  probably 
due  to  a  poisonous  grass  in  the  food  (vide  Scotsdale  guinea-pig).  Trypanosomes 
were  not  seen  on  autopsy,  and  the  body  was  well  nourished. 
Guinea-pig  4.  An  animal  which  had  resisted  infection  by  T.  vivax  a  year 
previous.  ^ 
September  24th,  igo8.  Inoculated  intraperitoneally  with  three  drops  of  blood 
from  Dog  i.  The  animal  remained  in  perfect  health,  and  trypanosomes  were 
never  seen  at  frequent  (every  two  days)  examinations  up  to  November  cth. 
Organisms  were  first  seen  on  November  5th,  and  were  thence  present  at  most 
subsequent  examinations  till  death  on  December  17th,  which  was  without  doubt 
accelerated  by  frequent  exposure  to  the  rains  and  cold  weather  and  by  constant 
travelling. 
Dog  I.  young  native  bitch.  Inoculated  subcutaneously  September  cth, 
1908,  with  twelve  drops  blood  of  original  case.  Organisms  were  not  seen  on 
September  12th,  the  only  examination  prior  to  arrival  at  camp,  September  22nd. 
Torn  this  date  to  that  of  death,  October  i8th  (thirty-three  days),  trypanosomes 
were  constantly  present.  Beyond  emaciation  and  weakness  no  special  symptoms 
were  observable— there  was  no  corneal  opacity,  as  in  the  original  animal.  The 
temperature  was  constantly  elevated  to  102°  to  103°,  and  showed  no  paroxysmal 
tendency.  ■' 
Dog  6.  October  30th,  igo8.  Received  6-oc.c.  subcutaneously  of  this  strain 
passed  through  Ox  i,  trypanosomes  1-4  fields.  It  did  not  become  infected,  and 
died  of  pleuro.pneumonia  on  December  21st. 
Dog  II.— November  ist,  igo8.  Inoculated  subcutaneously  with  twenty  drops 
flood  from  Rat  1.  Temperature  commenced  to  rise  on  the  fifth  day,  and  trypano¬ 
somes  one  to  a  field  were  seen  on  the  sixth.  During  the  time  it  was  taken,  the 
einperature  was  considerably  elevated,  reaching  io5'4°,  and  trypanosomes  were 
constantly  present  till  death  on  December  loth  (forty  days). 
Dog  13.— This  dog  served  as  a  control  to  Dog  6,  which  did  not  become 
mtected  after  inoculation  from  Ox  i.  November  gth,  igo8.  Inoculated  intra- 
peritoneally  with  30C.C.  from  Ox  i  (trypanosomes  1-30  fields).  The  temperature 
ommenced  to  rise  on  the  fifth  day,  and  trypanosomes  were  seen  on  the  seventh 
and  at  succeeding  examinations  up  to  death  on  November  27th— 18th  day. 
G  Inoculated  subcutaneously  with  heart  blood  of 
were  very  numerous  at  the  first  examination  on  the 
®  '  The  disease  was  acute  and  the  dog  died  on  December  31st — i6th  day 
