360 
Sheep  2. — September  29th.  Inoculated  subcutaneously  with  three  drops  blood 
of  Dog  I.  Trypanosomes  one  to  a  cover  were  seen  on  the  eleventh  and  twelfth 
day,  when  the  animal  was  destroyed. 
Sheep  7. — November  14th.  Inoculated  intraperitoneally  with  lo-o  c.c.  blood 
from  Ox  I.  The  object  of  this  experiment  was  to  note  whether  the  morphological 
forms  present  in  this  Ox  would  reappear.  Trypanosomes'  i  in  cover-glass  were 
seen  in  the  blood  on  one  occasion  only  (December  15th)  ;  they  were  present  in  the 
glands  on  December  ist,  loth  and  23rd,  and  not  seen  on  December  14th.  This 
animal  died  in  the  train  on  January  8th,  and  was  too  decomposed  for  inoculation 
on  arrival  in  Pretoria  the  following  day. 
Goat  i. — An  adult  female.  September  30th.  Inoculated  subcutaneously  with 
three  drops  blood  from  Dog  i.  The  temperature  rose  on  the  sixth  day  and  again 
on  the  tenth  (io6-8°),  from  which  date  it  was  almost  continually  elevated  above 
104°,  and  after  the  fifth  week  was  over  105°.  Trypanosomes  were  only  seen 
twice  on  direct  examination  of  the  blood — i  to  half  a  cover-glass  (^-inch)  on 
each  occasion —  the  i8tlh  and  27th  day.  They  could  constantly  be  found  on 
puncture  of  the  prescapular  glands.  No  symptoms  were  noted  beyond  slight 
emaciation,  the  animal  retaining  its  strength  until  it  was  destroyed  on  our  leaving 
for  home. 
Goat  4. — Adult  female.  October  30th  was  inoculated  subcutaneously  with 
4-0  c.c.  blood  from  Ox  1.  The  temperature  commenced  to  rise  on  the  fourth  day 
and  continued  irregular,  but  alway'S  elevated  between  103°  and  107°,  till  it  was 
destroyed  on  November  15th.  Trypanosomes  were  never  seen  in  the  peripheral 
blood;  but  they  were  constantly  present  in  the  prescapular  gland  from  the  seventh 
day. 
Ox  I. — A  five-year-old  cow.  Had  been  under  observation  for  eighteen  days 
prior  to  inoculation,  during  which  she  was  in  perfect  health  and  her  temperature 
had  never  exceeded  102°. 
October  i8th,  was  inoculated  subcutaneously  with  8'oc.c.  from  Dog  i,  just 
dead.  The  temperature  commenced  to  rise  the  following  day  (io3'2°).  On  the 
third  day  it  was  105°,  and  106°  on  the  fourth  after  inoculation,  and  then  fell  to 
103-2°.  For  a  week  it  continued  irregular,  showing  a  daily  variation  of  two  to 
three  degrees,  later  becoming  more  even,  but  ranging  daily  from  101-5°  to  over 
103°.  Trypanosomes  were  first  seen  i  to  half  a  cover-glass  on  October  24th — the 
sixth  day — and  they  were  thereafter  continually  present,  averaging  one  to  ten  fields  : 
on  two  occasions  only  did  they  exceed  one  to  a  field.  There  was  therefore  no 
periodicity  in  either  temperature  or  organisms;  and  no  increase  in  numbers  when 
a  high  temperature  (106-2°)  was  registered  early  in  the  infection.  Gland  puncture 
showed  trypanosomes  two  days  prior  to  their  appearance  in  blood.  During  the 
four  weeks  of  disease  she  was  under  observation,  there  was  noticeable  emaciation, 
the  animal  being  in  very  good  condition  at  the  time  of  inoculation.  At  our 
departure  she  was  left  under  the  charge  of  a  neighbouring  European  ;  but  up  to 
date  we  have  no  report  as  to  her  condition. 
A  suspicion  was  aroused  that  by  some  means  a  mixed  infection  might  have 
occurred,  and  a  series  of  inoculations  was  carried  out  to  ascertain  this  point.  We 
were,  however,  unable  to  obtain  any  evidence  of  such  an  occurrence. 
Ox  3. — ij  years  old.  This  Ox  was  inoculated  to  see  whether  the  trypano¬ 
somes  noted  in  Ox  i,  which  were  suspected  as  being  foreign  to  the  inoculation, 
would  again  reappear. 
October  31st.  Inoculated  subcutaneously  with  20  drops  blood  from  Rat  i. 
There  was  a  slight  thermal  reaction  sixth  and  seventh  days — from  then  to  the 
fifteenth,  the  day  we  left,  it  continued  normal.  No  trypanosomes  were  seen  in 
the  peripheral  blood  during  this  period,  but  gland  puncture  was  positive  from 
the  seventh  day.  We  have  no  report  as  to  the  present  condition  of  this  Ox. 
