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large  and  ro  /i  from  the  end,  but  frequently  it  is  almost  terminal; 
the  nucleus  is  compact,  round  or  somewhat  oval,  and  situated  towards 
the  middle  of  the  body.  The  undulating  membrane  is  represented, 
as  in  r.  vivax  and  most  of  the  long  forms  of  T.  nanum,  by  a  narrow’ 
band  extending  parallel  to  the  marg-in  of  the  body ;  its  rim  is 
continued  as  a  free  flagellum  of  from  q'o  to  6‘0  jx. 
In  its  morphology  this  trypanosome  so  closely  resembled  that 
which  we  described  as  T .  vivux  at  Broken  Idill  that  we  suspected  a 
mixed  infection  a  suggestion  accentuated  by  the  rapid  rise  in 
temperature  after  inoculation,  and  by  the  failure  to  infect  Dog  6. 
T.  vivax  was  not  rare  in  the  blood  of  inoculated  sheep  and  goats, 
while,  as  indicated  by  Sheep  2  and  Goat  i,  the  Chunga  organism  was 
uncommonly  seen.  Goat  4,  Sheep  y  and  Dog  i  3  were  inoculated 
from  this  cow  ;  the  two  ruminants  became  infected  without  showing 
numerous  trypanosomes  in  the  blood  (observation  on  Goat  4  limited 
to  fifteen  days  ;  no  peripheral  organisms  seen,  though  gland  puncture 
positive) ;  the  dog  became  infected  with  trypanosomes  identical  to 
those  seen  in  the  original  and  sub-inoculated  dogs,  the  short  forms 
markedly  preponderating.  As  a  control  to  these  experiments  a  second 
ox  was  inoculated  with  this  strain  passed  through  a  rat.  Unfortun¬ 
ately  we  had  to  leave  for  home  before  this  animal  showed  peripheral 
trypanosomes  ;  but  a  comparison  of  forms  seen  in  gland-puncture 
specimens  from  these  two  bovines  did  not  reveal  any  appreciable 
differences.  We  are  therefore  led  to  consider  it  possible  that  the 
Chunga  strain  passed  into  a  bovine  may  manifest  this  morphological 
variation,  and  that  the  failure  to  infect  Dog  6  was  due  to  individuality. 
Diagnosis 
Owing  to  lack  of  comparative  study  we  are  unable  to  assign  this 
trypanosome  to  any  particular  species.  In  our  original  dog  and  in 
several  of  the  sub-inoculations  it  shows  considerable  affinity  in  both 
morphology  and  animal  reaction  with  the  T.  evansi  group,  and  we 
have  spoken  of  it  as  allied  to  T.  hriicei.  In  the  strain  of  T .  brucei 
maintained  at  Runcorn,  forms  corresponding  to  both  ‘  long  ’  and 
‘  short  ’  are  found — the  so-called  '  male  ’  and  ‘  female  ’ — but  on  an 
average  they  are  larger,  and  more  transitional  stages  can  be  made 
out.  It  will  at  once  be  remarked  by  one  conversant  with  Laveran's 
