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368 
these  animals,  and  which,  as  we  have  already  noted,  may  be  a 
great  source  of  danger. 
An  enquiry  into  the  morphology  and  animal  reactions  of  the 
trypanosomes  met  with,  and  a  comparison  with  those  on  our  camp 
previously,  might  aid  towards  a  possible  solution.  We  are  only  able 
to  give  a  summary  of  the  few  reactions  obtained  piior  to  the  arrival 
of  the  strain  in  Pretoria ;  it  being  our  main  object  to  preserve  it  for 
experimentation  in  suitable  surroundings. 
Experimental. 
All  inoculations  made  direct  from  the  pig. 
g. _ October  31st.  Inoculated  intraperitoneally  with  S’o  c.c.  blood.  No 
trypanosomes  were  ever  seen,  and  this  animal  arrived  at  Pretoria  in  good  condition. 
Guinea-pig  ii. — October  31st.  Inoculated  intraperitoneally  with  5-0  c.c. 
blood  Trypanosomes  were  never  seen.  It  died  on  December  19th,  probably  from 
exposure.  There  were  no  indications  of  trypanosomes  on  post-mortem. 
Dog  10.— October  31st.  Inoculated  intraperitoneally  with  5-0  c.c.  blood. 
During  two  weeks  of  observation  the  temperature  remained  normal,  and  organisms 
were  not  seen  on  blood  or  gland  examination.  On  the  march  they  were  not 
encountered  at  various  examinations.  Death  took  place  from  pneumonia  on 
January  3rd.  There  were  no  indications  of  trypanosomes  on  post-mortem. 
QOA.T  6— October  31st.  Inoculated  subcutaneously  with  5-0  c.c.  blood.  The 
temperature  rose  to  106-4°  on  the  fifth  day,  and  for  the  remaining  ten  days  of 
observation  assumed  the  type  met  with  in  Broken  Hill. 
Trypanosomes,  one  in  five  fields  appeared  on  the  eighth  day,  were  absent  on 
the  ninth  and  tenth  (temperature  low),  were  seen  two  in  quarter  cover-glass  on 
the  eleventh  (temperature  107-2°),  absent  the  twelfth  (temperature  low),  and  were 
again  present  on  the  next  thermal  elevation  on  the  13th  day.  There  consequently 
appeared  to  be  a  connection  between  temperature  and  presence  of  organism. 
This  goat  travelled  well,  and  arrived  in  Pretoria  in  good  condition.  Trypano¬ 
somes  were  not  seen  in  the  peripheral  blood  on  the  journey,  but  a  gland  puncture 
on  December  21st  showed  them  still  present.  At  Pretoria  (January,  1909)  a  rahhit, 
guinea-pig  and  a  sheep  were  inoculated,  and  on  April  26th  Dr.  Theiler  informs  us 
that  the  original  goat  is  still  alive,  that  the  sheep  is  infected,  but  the  rahbit  does 
not  show  anything. 
Morphology  of  the  ‘Pig'  Trypanosome 
The  trypanosomes  present  at  death  of  the  original  host  were  so 
numerous  that  no  details  of  movement  could  be  made  out ,  this  more 
especially,  too,  since  they  were  agglutinating.  In  the  goat  they  could 
be  seen  crossing  the  field  without  difficulty,  giving  the  impression  of 
a  gliding  motion. 
In  stained  preparation,  (i)  Pig.  The  trypanosomes  measure 
from  1 1 ’5  to  17-9  /«  in  length  and  from  i  to  r8  /r  in  breadth.  The 
..  t  * 
