370 
blood,  which,  from  the  acuteness  of  the  pig’s  sickness,  might  be 
considered  of  a  virulent  nature. 
Our  observations  are  too  limited  to  identify  this  trypanosome  with 
certainty  as  T.  nanum,  which  is  characterised  by  negative  features; 
but  we  incline  to  the  opinion  that  future  work  will  show  they  are 
closely  related. 
We  can  now  consider  the  question  of  where  this  pig  derived 
infection,  and  at  once  a  local  origin,  a  transmission  from  the 
Scotsdale  strain  which  was  being  maintained,  will  suggest  itself, 
T.  nanum  was  not  found  locally  and  was  unknown  around  Abercorn ; 
and  though  its  existence  there  cannot  be  denied,  and  is  on  some 
grounds  to  be  suspected,  it  appears  more  probable  that  this  pig 
acquired  the  disease  after  arrival  at  Kambole,  where  Siomoxys  and 
Jlaematafota  were  common. 
VII.  EXAMINATIONS  OF  GAME 
Wherever  possible  an  examination  was  made  of  all  wild  animals 
ranking  as  game  which  we  shot,  or  which  had  been  killed  in  the 
vicinity  of  our  camps  by  others. 
In  most  cases  this  consisted  of  examining  the  blood  taken  from 
the  ear  or  heart  in  fresh  cover-glass  preparation  ;  but  latterly  gland 
juice,  and  sometimes  also  that  of  other  organs,  was  also  examined. 
In  no  instance  was  blood  or  other  fluid  centrifuged.  Inoculations 
into  small  animals  were  only  made  from  those  showing  flagellates  and 
from  a  wart-hog  and  a  buffalo  shot  by  Dr.  Yale-Massey  close  to  our 
camp  at  Broken  Hill.  Dry  slides  were  made  from  some,  and  were 
examined  stained  for  the  presence  of  other  blood  parasites  such  as 
piroplasma  and  spirochaeta.  These  have  not  yet  been  properly 
examined,  but  no  findings  are  recorded  up  to  date. 
The  majority  of  these  animals  were  killed  at  considerable 
distances  from  camp,  and  several  hours  elapsed  before  examination; 
but  not  a  few,  as  for  example  those  shot  on  the  march,  came  under 
the  microscope  within  an  hour. 
Bruce  has  indicated  that  direct  examination  is  of  small  value; 
but  the  positive  findings  of  Dutton  and  Todd  and  ourselves  show  that 
peripheral  organisms  may  be  present.  The  ideal  system,  however, 
would  be  to  establish  temporary  camps  hi  various  districts  with  a 
