37^ 
Experi.'XEN'I.— The  seium  of  guiacii-pig  3  was  obtained  shortly  before  the  death  of  the 
animal.  It  had  not  the  slightest  activating  action  even  -wheu  added  undiluted  to  the 
^tandardised  haemolytic  system  of  goat’s  red  cells  and  amboceptor.  A  portion  of  this  serum 
was  added  in  varying  jn-oportions  to  the  serum  of  a  normal  guinea-pig.  The  resulting 
mixtures  were  then  kept  at  37°  C.  for  20  minutes  before  being  added  to  the  haemolytic 
system.  A  second  portion  of  the  serum  of  guinea-pig  3  was  lust  heated  to  56°-58°  C.  for 
half  an  hour  in  order  to  destroy  any  complement  it  might  contain,  and  then,  as  aliove,  mixed 
in  different  proportions  with  normal  guinea-pig  serum. 
The  following'  Table  g-ives  the  result  of  this  experiment: 
A 
I 
:  0 
a  /o_ 
suspension 
of  goat’s  red 
cells 
Complement 
.Amboceptor 
Normal 
guinea-pig 
serum 
o-9%NaCl 
solution 
Haemolysis 
1  1‘0  cx. 
- 1 
3 
*  ■■  1 
0-002  c.c. 
?  1 
o-i  c.c. 
0-05  c.c. 
0-025 
o-oi  c.c. 
0*1  c.c. 
0’i5  c.c. 
0’T75  c.c. 
0*19  c.c. 
Complete  haemolysis  in  1 5  mins. 
Complete  haemolysis  in  30  mins. 
Partial  haemolysis  in  3  hours 
Trace  of  haemolysis  in  3  hours 
15 
5%. 
suspension 
of  goat's  red 
cells 
.Amboceptor 
CO.MPI.EMENT 
Normal 
guinea-pig 
serum 
Serum 
guinea-pig 
3 
Haemolysis 
I  i-oc.c. 
0-002  c.c. 
o-i  c.c. 
O'l  c.c. 
Complete  haemolysis  in  15  mins. 
2 
0-05  c.c. 
0-15  c.c. 
Complete  haemolysis  in  30  mins. 
3 
0-025  c.c. 
C-175  c.c. 
Partially  complete  haemolysis  in 
, 
3  hours 
4 
i 
o-oi  c.c. 
0-19  c.c. 
Slight  haemolysis  in  3  hours 
C 
CoMPLF.MEXT 
5  %. 
suspension  '  .Amboceptor 
of  goat's  red 
cells 
1 
Normal 
guinea-pig 
scrum 
Scrum  of 
guinea-pig 
3  ’  ' 
(heated 
56-58°  C. for 
20  mins.) 
Haemolysis 
I  1-3  c.c.  0-002  c.c. 
O' I  c.c. 
O'l  c.c. 
Complete  haemolysis  In  15  mins. 
0-05  c.c. 
0-15  c.c. 
Complete  haemolysis  in  30  mins. 
,, 
0-025 
0-175  c.c. 
Partial  haemolysis  in  3  hours 
4  M 
O'OI  c.c. 
. 
0-19  c.c. 
Trace  of  haemolysis  in  3  hours 
