SEROTHERAPY OF TETANUS. 
457 
shown that they possess an activity nearly alike ; at the dose of 
o gramme 000,000,000,1 (one ten-billionth of a gramme) both 
serums protect a mouse against a dose of toxin ten times supe¬ 
rior to the killing dose and a hundred times superior to the tetan- 
igenousdose. The Pasteur serum has proved itself a little more 
active than that from Hochst, as in the last experiments, several 
mice treated with the last serum have had tetanus of the inocu¬ 
lated leg, while those treated by the other serum remain im¬ 
mune. 
The Pasteur serum having an activity at least equal to that 
from Hochst, I was able to multiply my experiments. 
March 14, 1897, at 10 o’clock, I was shown four horses, 
which the 9th of the same month had received under the skin 
of the neck 6 milligrammes of dry toxin from Pasteur’s. One 
of them (No. 60,738) was evidently tetanic ; in the morning, at 7, 
he was found with his tail elevated, his neck stiff, and he moved 
as one piece ; he will serve as a witness. 
The three others (52,824, 45,465, 57,624) presented nothing 
abnormal at 7 o’clock. At 10 they were examined again, their 
movements were stiff; I considered them taken and injected 
them in the jugular; to the first I gave 10 grammes of dry 
serum of Pasteur dissolved in tepid water and representing 100 
cubic centimetres of liquid serum ; the other two received 100 
cubic centimetres of liquid serum. 
March 15 the four horses were very sick, their legs as stiff 
as posts, trismus well marked, appetite gone. 
The witness, the 52,824 and 45,465, had fallen, and on the 
16th the tetanus was generalized, dying during the night. 
In No. 57,624 the disease had marched more slowly ; it was 
generalized only on the 17th, the horse fell on the i8th and 
died on the 19th. 
Therefore the intravenous injection of antitetanic serum 
made as soon as the appearance of the prodromes of tetanus is 
powerless in arresting the fatal progress of the disease, even 
when the injected d'ose is double that indicated as curative. 
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