SEROTHERAPY OF TETANUS. 
459 
March 9th, at 10.30 A. m., I injected under the skin of the 
neck, 6 milligrammes of dried toxin to five horses, Nos. 42,153, 
45 ) 55 o> 49 ) 645 ) 54)40? and 55,718. 
March 14th, 10 A. M., none of the horses, examined indi¬ 
vidually and closely, presented the slightest symptoms of lock¬ 
jaw. To the first three I injected, in the jugular, 100 cubic 
centimetres of Pasteur antitetanic serum (obtained from horse 
No. 4 on February 15th) ; the two other horses were kept as 
witnesses. 
March 15th the five horses presented some suspicious symp¬ 
toms : in the stable a little elevation of the tail could scarcely 
be observed; when taken out they showed stiffness of the neck 
and extremities. The i6th tetanus was well marked in all. 
They all still ate. The 17th general contractions; tail ele¬ 
vated ; neck curved; nostrils dilated; prehension of food 
almost impossible. The i8th they were still worse ; about 10 
A. M., No. 45,153 fell, struggled, and died in the evening ; one 
witness (No. 54,407) died the 19th, at 4 A. M.; the other in the 
evening towards 5 p. M.; the two others fell on the 20th and 
died the same day. 
Therefore, the intravenous injection of a large dose of anti¬ 
toxic serum is powerless in arresting the development of teta¬ 
nus, resulting from a dose of toxin, always fatal to witness, 
even when this injection is made twenty-four hours before the 
appearance of the first symptom. 
* * 
If interference is applied 48 hours before, there are many 
chances for the patient to escape ; sometimes even the appear¬ 
ance of any tetanic symptom is avoided; but the injection 
must be made with large doses of serum, introduced in the 
veins. 
(i) PTbriiary 24, 1897, I injected 6 milligrammes of dried 
toxin under the skin of the neck of two horses. Nos. 46,214 and 
49 ) 344 - 
February 28, in the jugular of the first, I injected 100 
cubic centimetres of Pasteur serum (obtained February 15th 
