460 
E. NOCARD. 
from horse No. 4). The other inoculated horse was kept as 
witness. 
March 2d, 12 o’clock, the witness had certainly taken ; his 
tail was elevated ; there was stiffness of the neck and loins. 
March 3d, tetanus generalized ; abdomen retracted; legs stiff 
as posts; nostrils dilated; head extended on contracted neck; 
he died during the night. 
The horse on treatment escaped all tetanic symptoms. 
(2) March 9th, a Danish horse (No. 54,096) received, under 
the skin, with 13 other horses, 6 milligrammes of toxin. 
On the 13th, he received, the skin^ 60 grammes of 
Pasteur serum (obtained February 15th from horse No. 4). 
Two other horses, (Nos. 54,407 and 55,178), left as witnesses, 
were taken only on the 15th of March. Therefore, the treat¬ 
ment preceded the appearance of tetanus by 48 hours. Never¬ 
theless, on the 16th, .the treated animal was effected; the i8th, 
the disease was generalized, he died March 21st, two days after 
the witness. 
The dose of serum injected might have been sufficient to 
save the animal, if the injection had been made in the jugular 
instead of under the skin. 
* 
* * 
A last series of experiments show well the great preventive 
value of the antitoxic serum ; a very weak dose of serum is 
sufficient to prevent the appearance of tetanus, when the injection 
is made, even under the skin, a short time after the animal has 
received the dose of toxin, always fatal for the witnesses. 
March 9th, 10 a. m., under the skin of the neck of fourteen 
horses I injected 6 milligrammes of toxin: of these, three not 
treated and used as witnesses became tetanic, one (Hungarian) 
March 14th, the two others (Danish) the 15th. Seven others, 
treated either at the moment of the appearance of the first 
symptoms, either twenty-four or forty-eight hours before, con¬ 
tracted a fatal tetanus. 
The four last resisted, thanks to a more early treatment. 
No. 46,443 (Norman) received under the skin 10 c.c. of Pasteur 
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