SOME REMARKS ON ANTI-HOG CHOLERA SERUM. 
677 
time. That such a poison, or a similar one, at least is present 
in the organs of animals dead from hog cholera infection, is 
easily shown by grinding up their spleen, and extracting it with 
a ten per cent, glycerine solution ; the resulting fluid is of a 
fairly high toxic potency, as shown in the animal experiment. 
There is no doubt, in my mind, that these facts reinforce the 
toxic theory ; the individual case may, of course, offer predomi¬ 
nating the clinical picture of septicsemia. 
That it is an easy thing by means of cultures and culture 
products to immunize small animals, has already been shown by 
Salmon. But nobody as yet has shown, that the blood of such 
animals has protective or curative properties. Perhaps this is 
due to the fact that it is very difficult, in rabbits at least, to es¬ 
tablish a tolerance for high doses of the living bacillus. They, 
too, are very susceptible for its poisonous products; continued 
injections produce some kind of a maranthic condition in them 
which it is impossible to overcome. 
Even in higher animals this is the imminent danger, and 
great care must be exercised in the dosage and increase of the 
injections. P'or my experiments I used a donkey and horses. 
The former had soon to be rejected on account of nasty and 
deep-seated abscesses which regularly formed after each injec¬ 
tion, and greatly helped to reduce its physical strength. 
On the horses, too, the treatment had an exhaustive tendency. 
I began with a small dose (i c. c.) of toxin (filtered 6 weeks 
culture) given hypodermically, and increased it in the course of 
3 months until 500 c. c. were given at one injection. The in¬ 
jections were given about every week. The reactions were 
quite severe, general as well as local. The fever ran up to 
104° and 10414° F. for several days, and at the site of the in¬ 
jections enormous oedemate and gelatinous infiltrations formed. 
It was peculiar that the latter did not grow less as the treatment 
progressed. After these high toxin doses had been reached, liv¬ 
ing 48-hours old cultures were injected, and up to 200 c. c. were 
given. In contradistinction to non-treated animals, these cult¬ 
ures were borne nicely, and did not produce any abscesses. The 
