824 
VERANUS A. MOORE, B.S., M.D. 
Bacillus of hog cholera. 
1. Rod-shaped organism with ends 
rounded, 1.2 to 2.0a in length, 0.5 to o.8m 
in width. The size varies according to the 
stage of growth and division, and the culture 
media. 
2. From cultures it stains entirely. In 
tissues it usually stains around the periphery 
with darker extremities leaving a light 
centre. 
3. Actively motile in liquids. 
4. From 3 to 9 flagella are demonstra¬ 
ble. 
5. Vigorous growth in alkaline nutrient 
liquids. Less vigorous if liquids are acid 
in reaction. 
6. Moderate growth on potato. (Varies 
according to reaction.) 
7. Distinct growth on gelatin. 
8. Saponifies milk in from 3 to 4 weeks. 
9. Ferments dextrose with the formation 
of acids and gas. 
10. Does not ferment lactose. Bouillon 
containing it becomes strongly alkaline. 
No gas. 
11. Does not ferment saccharose. 
Bouillon containing it becomes strongly 
alkaline. No gas. 
12. Destroyed by moist heat at 58° C. in 
15 minutes. 
13. Dies in water in from 2 to 4 months. 
14. It dies in the soil in from 2 to 3 
months. 
15. Rabbits injected subcutaneously 
with o.i c.c. of a bouillon culture of a 
virulent bacillus will die in from 5 to 7 
days. Enlarged spleen, necrotic foci in 
liver. 
16. Rabbits inoculated with culture of 
an attenuated variety live from lo to 20 
days or recover. The lesions are enlarged 
spleen, and infiltration of the follicles in 
Beyer’s patches. 
17. In guinea-pigs the lesions are prac¬ 
tically the same as in rabbits. Death oc¬ 
curs in from 7 to 12 days. 
Bacillus of stuine plagtie. 
1. Elongated oval organism 0.8 to 1.5/i 
in length, 0.6 to 0.8 in thickness. The 
size varies according to the stage of growth, 
and division, and the culture media. 
2. From old cultures it usually stains en¬ 
tirely. When in process of division as 
found in the organs ot freshly dead rabbits 
the extremities stain leaving an unstained 
central band, “ polar stain,” 
3. Not motile in liquids. 
4. No flagella have been found. 
5. Growth moderate or feeble in alkaline 
nutrient liquids. No growth if liquids are- 
acid. 
6. No growth on potato. 
7. Feeble or no growth on gelatin. 
8. Produces no apparent change in milk. 
9. Ferments dextrose with the formation 
of acids but no gas. 
10. Does not ferment lactose. No gas. 
11. Ferments saccharose with the forma¬ 
tion of acids. No gas. 
12. Destroyed by moist heat at 58° C. 
in 7 minutes. 
13. Dies in waterin from 10 to 15 days. 
14. It dies in the soil in from 4 to 6 
days. 
15. Rabbits injected subcutaneously with 
o.oi c.c. of a bouillon culture of a virulent 
bacillus will die in from 16 to 20 hours. 
Septicsemia. 
16. Rabbits inoculated with a culture 01 
an attenuated variety will live from 4 to lo 
days. The lesions are local infiltration of 
pus cells with pleuritis, pericarditis or peri¬ 
tonitis. 
17. Guinea-pigs are slightly less suscep¬ 
tible than rabbits. There is more local re¬ 
action. Death occurs in from i to 4 days. 
