A Bacterial Disease of Alfalfa 13 
the protoplasm, but no definite meta-chromatic or polar gran¬ 
ules are demonstrable. 
II. CULTURAL FEATURES 
1. Agar Stroke. Nutrient Agar. 
GROWTH, at 27°-28° C., moderate in 24 hours, abundant after 
72 hours; no growth at 37° C. 
FORM OF GROWTH, filiform becoming more or less echinulate 
with age. 
ELEVATION OF GROWTH, slightly convex. 
LUSTER, glistening. 
TOPOGRAPHY, smooth. 
OPTICAL CHARACTERS, translucent. 
CHROMOGENESIS, § grayish white, Engine color No. 12. 
ODOR, absent. 
CONSISTENCY, butyrous. 
MEDIUM, light fluorescent green on the third day. 
2. Potato. 
GROWTH, scanty in 4 8 hours, moderate after 6 days. 
FORM OF GROWTH, filiform becoming more or less echinulate 
with age. 
ELEVATION OF GROWTH, slightly convex. 
LUSTER, glistening. 
TOPOGRAPHY, smooth. 
CHROMOGENESIS, cream; best described as the color of light 
manilla paper; color lies between Orange Yellow Tint No. 2 
and Engine Color No. 1. After 8 days, the color deepens to 
Orange Yellow Tint No. 2, and after 12 days, resembles most 
nearly Engine Color No. 7. 
ODOR, absent. 
CONSISTENCY, butyrous and slightly viscid. 
MEDIUM, grayed and darkened. 
3. Loeffler’s Blood Serum. 
STROKE, moderate in 3 days at 28° C. 
FORM OF GROWTH, filiform. 
ELEVATION, slightly, convex. 
LUSTER, glistening. 
TOPOGRAPHY, smooth. 
CHROMOGENESIS, grayish white. 
4. Agar Stab. 
GROWTH, best at top; surface growth moderate in 48 hours, 
abundant after 4 days; surface growth restricted. 
LINE OF PUNCTURE, papillate; no liquefaction. 
CHROMOGENESIS, grayish white, Engine Color No. 12. 
MEDIUM, fluorescent at the surface on the third day. 
5. Gelatin Stab. 
GROWTH, best at top, no growth in the lower half of the stab. 
LINE OF PUNCTURE, filiform to papillate. 
LIQUEFACTION, none. 
MEDIUM, unchanged. 
6. Nutrient Broth. 
SURFACE GROWTH, pellicle on the third day. 
CLOUDING, slight in 2 4 hours, moderate in 4 8 hours, never 
strong; persistent; fluid slightly turbid. 
ODOR, absent. 
SEDIMENT, compact, slightly viscid on agitation, scant. 
§ Color terms refer to Standard Colored Papers made by Milton 
Bradley Co., Springfield, Mass. 
