' 
A Bacterial Disease oe Alfalfa 15 
ODOR, none. 
CONSISTENCY, butyrous. 
MEDIUM, surface becomes clouded as if a very fine precipitate 
had been formed in the medium as a result of the growth. 
13. Synthetic Agar Low in Nitrogen. Agar stroke. 
GROWTH, at 27°-28° C., moderate in 24 hours; very abundant 
after 3 days. 
FORM OF GROWTH, filiform. 
ELEVATION OF GROWTH, decidedly convex. 
LUSTER, glistening, watery. 
TOPOGRAPHY, smooth. 
OPTICAL CHARACTERS, transparent; resembles boiled starch. 
CHROMOGENESIS, watery white. 
ODOR, none. 
CONSISTENCY, butyrous or more exactly the consistency of 
starch jelly. 
MEDIUM, no change. 
14. Colin’s Solution. 28° C. 
GROWTH, absent. 
FLUID, no growth. 
15. Uschinsky’s Solution, 28° C. 
GROWTH, slight in 2 4 hours, moderate in 3 days, abundant in 
10 days. 
FLUID, viscid sediment after 4 days; clouding, strong, persis¬ 
tent, fluid turbid. Light green after 21 days. 
16. Dunham’s Solution, 28° C. 
GROWTH, moderate. 
CLOUDING, moderate, persistent, fluid slightly turbid. 
17. Asparagin Solution, 28° C. 
GROWTH, abundant. 
CLOUDING, moderate, persistent, fluid turbid. 
18. Nitrate Broth, 28° C. 
GROWTH, moderate. 
CLOUDING, moderate, persistent, fluid slightly turbid. 
19. Dextrose Bouillon, 28° C. 
GROWTH, abundant. 
CLOUDING, strong, persistent, 
20. Saccharose Bouillon, 28° C. 
GROWTH, abundant. 
CLOUDING, strong, persistent, 
21. Lactose Bouillon, 28° C. 
GROWTH, abundant. 
CLOUDING, strong, persistent, 
22. Maltose Bouillon, 28° C. 
GROWTH, abundant. 
CLOUDING, strong, persistent, 
23. Glycerine Bouillon, 28° C. 
GROWTH, abundant. 
CLOUDING, strong, persistent, 
24. Mannite Bouillon, 28° C. 
GROWTH, abundant. 
CLOUDING, strong, persistent, 
fluid turbid. 
fluid turbid. 
fluid turbid. 
fluid turbid. 
fluid turbid. 
fluid turbid. 
25. Growth in Bouillon Over Chloroform. 
GROWTH, absent. 1 c.c. of chloroform was added with a 
sterile pipette to 10 c.c. of sterile bouillon in a test tube and 
after the chloroform had collected at the bottom of the tube, 
the broth -was inoculated with two loops of a 24 hour broth 
culture. 
