A Bacterial Disease of Alfalfa 23 
Sugar Broths. —These were prepared by adding one per cent 
of the different sugars, as well as glycerine and mannite, to sugar 
free broth. 
Glycerine Agar. —Prepared by adding four to six per cent 
glycerine to nutrient agar. 
Dunham's Solution .— 
Distilled water. ..1000.0 c. c. 
Witte’s Peptone. 10.0 grams 
NaCl. 5 0 grams 
Nitrate Broth .— 
Distilled water. 
Witte’s Peptone. . 
KNO s (nitrite free). 
Asparagin Solution. — (Jordan). (2) 
Redistilled water. 
Asparagin. 
MgS0 4 . 
Ko HPCL. 
.. 1000.0 c. c. 
1.0 gram 
.. .2 gram 
.. 1000.0 c. c. 
2 0 grams 
1.0 grams 
1.0 grams 
627. 
(1) Supplement Journal Infect. Diseases, May, 1905, p. 104. 
(2) Jordan. Bot. Gaz., 1899, 27, p. 9; Jour. Expt. Med., 1899, 4, p. 
UsGlinsky s Solution .— (i) 
Distilled water. 
Glycerine. 
NaCl. 
CaCl.,. 
MgSO, . 
Ko HP0 4 . 
Ammonium lactate . 
Sodium asparaginate. 
Cohns Solution. — (2) 
Distilled water. 
KHo PCL. 
MgS<L. 
Ammonium tartrate. 
KC1. 
1000.0 c. c. 
30-40 grams 
5- 7 grams 
. 1 grams 
.2-.4 grams 
2.-2.5 grams 
6 -7 grams 
3 -4 grams 
1000.0 c. c. 
5.0 grams 
6.0 grams 
10.0 grams 
. 5 grams 
Synthetic Agar f Loza in Nitrogen .— 
Distilled water . 1000.0 
Cane sugar. 50.0 
kh„po 4 . 1.0 
MgSCL.2 
Shredded agar . 15.0 
Reaction +3° Fuller’s scale. 
(1) Cent. f. Bakt., Erste Abt., Bd. XIV., 1893, p. 316. 
(2) DeBarry, p. 8 6, Vorles. u. Bact., 2 Auflage. 
c. c. 
grams 
gram 
gram 
grams 
Potato. —Cylinders of raw potato were washed in running 
water over night, rinsed in a very dilute solution of NaOH, and 
sterilized in the autoclave for five minutes at 120° C. 
Alfalfa Agar. —Prepared by adding 15 grains of finely 
shredded agar to 1,000 c.c. alfalfa infusion made by steeping 500 
grams finely chopped green alfalfa in 1,000.0 c.c. tap water for one 
hour. Reaction not changed. 
