22 
W. L. WILLIAMS. 
fested to supposedly healthy chick, by means of introduction of 
the living parasites into the air sacs. 
Subject, an apparently healthy incubator chick which had 
not been in contact with other than incubator chicks and on 
premises supposed to be free from cytodites. 
On Oct. 23, 1895, twelve living parasites from Case 6 were 
introduced into the air sacs of the experiment bird by means of 
intercostal incision as for caponizing. The subject was then 
kept apart from other birds except No. 8, and in quarters sup¬ 
posedly free from parasites. No symptoms of disease developed 
and after an interval of forty-seven days (December 9th) the 
ehick was killed by bleeding and the autopsy revealed a few 
cytodites in varying stages of development, including young 
mites and pregnant females. No trace of pathological lesions 
was discovered. 
Case No. 8. Experimental transmission of cytodites to 
healthy chick by laryngeal injection. Incubator chick with 
history of No. 7. About twelve living mites from No. 6, 
suspended in water, were injected through larynx into trachea, 
and chick cared for as No. 7, without the occurrence of signs of 
ill health other than those attributed to close confinement. 
Autopsy November 6, 1896. No cytodites could be found, 
and no pathological lesions distinguishable. 
Case No. 9. A one-year-old partridge Cochin hen, property 
of Experimental Station. Noted unwell about December 15,1895, 
being very weak, dull, rapid emaciation, quickened and diffi¬ 
cult breathing, and feathers much soiled, well marked diarrhoea 
present, the faeces thin, pale yellowish, stringy. Killed by 
bleeding, December 26, 1895. Autopsy: Diffuse enteritis, 
right anterior diaphragmatic air sac contained about 500 cyto¬ 
dites, right posterior diaphragmatic about 200, right abdominal 
sac 4 oz. of a pale yellowish serosity, slightly turbid. The air 
sacs on left side were not examined for some hours, when but a 
few parasites were found in them. No further pathological 
lesions were observed. 
Case No. 10. A brown Leghorn hen, aged three and one- 
