172 
VERANUS A. MOORE. 
chided within the possibilities of its etiological factor more 
clearly defined, the question of its identity or non-identity 
with the local infections here described cannot be answered. 
In both the disease seems to be due to local causes. 
Bacteriological Exa^nination .—As already stated, several 
agar cultures were made at the time of the examinations from 
the pus or indurated tissue from three of the cases. On the fol¬ 
lowing day bouillon and agar tubes were inoculated and gelatin 
and agar plate cultures were made. In addition to these several 
eultures were made on special media from the material obtained 
in sterile tubes. Without entering into wearisome details, the 
results of these cultivations from the different animals may be 
summarized as follows: 
From Case No. i.—A streptococcus and an undetermined 
micrococcus and bacillus. 
From Case No. 3.—A streptococcus and several (about six) 
forms of chromogenic microeocci and bacilli. (The foot in this 
case had been wrapped for several days in oakum wet with some 
disinfectant.) 
From Case No. 4.—A streptococcus which appeared in pure 
culture in most of the tubes. In three tubes bacillus colt 
communis was also present. 
In the plate cultures in agar made from the material from 
cases Nos. i and 4 colonies of the streptocoeciis predominated. 
In those prepared from case No. 3 colonies of chromogenic mi¬ 
crococci were more numerous. 
The culture of the eolon bacillus was carefully studied in 
both its cultural characters and its effect upon animals, but it 
did not reveal any properties uncommon to that species. This, 
together with the faet that it appeared in the cultures from 
but one case, suggests that its presence was aceidental and that 
it did not stand in any etiological relation to the inflammatory 
process. With the exception of the streptococcus, all of the 
other bacteria which appeared in the culture were common 
saprophytes which presumably had found their way into the 
open sores. 
