174 
VERANUS A. MOORE. 
The microscopic examination of cover-glass preparations 
from the pus taken from case No. 4 showed a streptococcus in 
short and long chains. In those made from the material from 
cases Nos. i and 3 there were in addition to the streptococcus 
several other forms of bacteria. The infiltrated subcutaneous 
tissue from case No. i was found to consist of round cell infil¬ 
tration. The fresh preparations of the pus from the other cases 
(Nos. 3 and 4) revealed nothing unusual for purulent material. 
Inoadation into Cattle ,—In order to determine the infectious 
nature of the trouble, two cows were inoculated with the ma¬ 
terial obtained from cases Nos. 3 and 4. A small portion of 
the pus from each specimen was diluted in sterile bouillon and 
about 2 c.c. of the suspension injected subcutaneously just above 
the hoof in the left fore foot in each case. Swelling was 
noticed on the third day. It gradually extended up the leg to 
the knee joint. There was much tenderness and evidence of 
pain. The subcutis became indurated in the lower part of the 
leg and on the loth and 12th days suppuration was evident. 
The abscesses were not opened, but a few days later they broke 
just under the dew-claws, near the place of inoculation, and dis¬ 
charged a considerable quantity of cream-colored pus, after 
which complete recovery soon followed. The streptococcus 
was obtained in pure culture from each of these cases. 
A third cow was inoculated with a pure bouillon culture 24 
hours old of the streptococcus obtained from case No. 4. The 
inoculation was made by scraping the skin on the right fore foot 
just above the hoof, and after removing the epidermis the cul¬ 
ture was rubbed on the raw surface. Swelling began in three 
days and the symptoms already described followed in regular 
order. A subcutaneous abscess formed and on the 14th day 
it discharged. The streptococcus was obtained in pure cultures 
from the freshly discharged abscess. Recovery rapidly followed. 
A cow was inoculated in the tail with a pure culture of the 
streptococcus with negative results. 
The Streptococcus ,—The cultures of streptococcus isolated 
from the different animals (cases Nos. i, 3 and 4) were care- 
