240 
FRANK H. MILLER. 
aspect and limpidity of the fluid can be admitted. Hence, in 
the supposition that this same microbe should belong to the 
class of those which are beyond the limits of visibility, the 
only criterions of its presence and growth by cultivation will 
be inoculation. 
Perhaps, already, some experimenters have obtained such 
cultures ; but they have overlooked them because, the fluid 
having kept its limpidity, they have thought it useless to inocu¬ 
late it. 
For this running of thoughts, cultivation in vivo^ with col¬ 
lodion or reed-cane bags, which has been so useful already, has 
not said its last word ; it has no doubt some other surprise in 
store for us. 
CONCLUSIONS. 
The agent of pleuro-pneiimonic virulency is constituted by 
a microbe, extremely small ; its dimensions, very much lower 
than that of the smallest known microbes, do not allow, even 
after coloration, to make out exactly its form. 
The microbe grows easily in bags of collodion or of reed- 
cane placed in the peritoneum of rabbits. 
It does not grow when inoculated in vitro in media of cul¬ 
ture ordinarily in use. 
On the contrary, it grows easily, when inoculated in the 
peptone-bouillon of Martin, to which serum of cow or rabbit is 
added in the proportion of one part of serum to twenty-five of 
bouillon. 
{To be continued.') 
[ Written specially for the American Veterinary Review. ~\ 
OBSERVATIONS MADE IN CANINE MEDICINE. 
“ CONJUNCTIVITIS FOLUICULARIS.” 
By Frank H. Miller, V. S., New York City. 
With a thorough appreciation of the multitudinous obliga¬ 
tions entailed upon the time and patience of the veterinarian 
who devotes himself to the practice of general veterinary medi- 
