382 
NOCARD AND ROUX. 
tration of the connective tissue has extended to the interfas¬ 
cicular tissue, in such a way that, on section, the muscle has 
the aspect of being divided in sections, it seems sclerous; only 
the connective tissue between its bundles of muscle are very 
cedematous ; between them, the tissue proper of the muscle has a 
pale washed color, and is soft in its consistency. This condition 
exists also in the intercostals ; the serous infiltration has spread 
into the sub-pleural connective tissue, where it forms a thick 
and fluctuating cushion. The same exists also in the cellular 
tissue of the anterior mediastinum. The pleural sac contains 
about two liters of yellow serosity, somewhat reddish. Both 
pulmonary lobes are healthy ; there is not the slightest inter¬ 
stitial or sub-pleural infiltration. 
* 
* * 
One might suppose that the result of this experiment is 
because it was only a simple dilution of the virus which had 
been injected ; this is not admissible. The original bouillon 
was inoculated with one drop of sub-pleural serosity, say 1—20 of 
a c.c. for 10 c.c. of bouillon ; the dilution then was at 1—200— 
5 drops from the liquid of the bag, diluted to 1-200, or i 
cubic centimecer of a dilution to 1-800; the inoculation has 
been- made forty days after the collection of the virus—that is, 
at a date where ordinarily the serosity has lost its virulency; 
let us observe, besides, that for twenty days the serosity, diluted 
to 1—200, has stood a temperature close to -[-40° (in the perito¬ 
neum of the rabbit), a condition most unfavorable to the preser¬ 
vation of virulence ; let us also say, that the incubation has been 
very short and the progress of the infection very rapid, and we 
will conclude that the results observed are due to the cultivated 
microbe, and not to a simple dilution of the pleuro-pneumonic 
virus. 
The following experiments will remove all doubts : 
Second Series of Experiments. —July 19, 1896, a great 
quantity of pure serosity, which is in the connective tissue of 
the left costal region of heifer No. i, is collected. 
August I, three collodion bags receive : one, fresh bouillon 
