384 
NOCARD AND ROUX. 
nary refringent points ; two new dilutions (to 1-200) are made 
with it, placed in new bags, and inserted in the peritoneum of 
rabbits B, 833 and B, 831. The balance of the dilution is placed 
in the thermostat and remains sterile. 
Rabbit B, 833 dies Sept. 29, in the morning, without ap¬ 
parent cause, cachectic; the bag is intact, the liquid very 
opal, contains only the ordinary small refringent points; it 
is placed in sealed tubes and kept until the death of rabbit 
B, 831. 
This animal is killed Oct. 6. He is very lean, but still 
strong. The bag is intact, it contains very little of a very 
cloudy liquid, with the small ordinary refringent points, cocco- 
bacilli analogous to that of chicken cholera, though smaller, are 
found quite numerous; there are no cells; undoubtedly they 
are due to an impurity introduced in the bag at the time it was 
filled and closed. 
October 8, i c.c. of the culture of rabbit B, 833 is injected 
under the skin of a cow (No. 3) back of the shoulder. 
Here is the resume of the observations of the two cows in¬ 
oculated in this series of experiments : 
Cow No. 2 (has received, July 7, i c.c. of a second culture in 
bag, representing a dilution to i—10000 of the serosity obtained 
from the heifer No. 2, July 19, 1896). 
Up to September 15, this cow presents nothing abnormal; 
16th, at the point of inoculation, is observed a hard, sore tume¬ 
faction, as big as a Mandarin orange ; temperature still normal; 
17th, swelling is larger ; tense, warm, very painful. 18, it meas¬ 
ures more than 20 centim. in diameter; temperature raised to 
39.3°. 20th, the swelling, very tense, has reached the shoulder, 
whose motions are stiffened ; temperature is 40.2° ; animal is 
dull and refuses part of her ration ; injections of iodine water 
are made in the oedematous engorgement surrounding the 
tumor. Up to the 23d, the condition remains the same, always 
serious ; injections of iodine are continued. From the 24th, the 
tumor began to diminish, the temperature subsides, the appetite 
returns. The 27th, everything is normal. 
