386 
NOCARD AND ROUX. 
No.j. —Cow, 5 years old, inoculated Oct. 8, 1896, with i c.c. of bag 
culture of rabbit B 833 (dilution 1-40,000,000.) 
December ii, 1896, two lungs of pleuro-pneumonic bovine 
were sent to Alfort: the right lobe in its posterior half is the 
seat of a recent hepatization ,; the tissue is gorged with yellowish 
and limpid serosity ; some cubic centimeters of it are collected 
(pure) which will serve to inoculate by injection under the skin, 
back of the shoulder, cows No, 2 and 3 ; each receives twenty 
drops of serosity, and cow No. 4 (Normandy, 18 months old, with 
actinomycosis of the jaw), which will be zvitness^ receives only 
10 drops of serosity. 
While both cows. Nos. 2 and 3, have resisted the injection 
without presenting anything abnormal, no swelling, nor even 
temporary fever, the zvitness died the 2 2d day with an enormous 
swelling containing more than 10 litres of serosity. The in¬ 
cubation has been only eight days; December i8th, the fever 
started and the oedematous swelling at the point of inoculation 
made its appearance. 
Third Series of Experiments. —March 9, 1897, sub- 
pleural serosity is collected pure from a cow killed. On the 12th, 
two collodion bags are prepared and filled with a diluted solu¬ 
tion of serosity in peptone bouillon to the i-iooo. One bag is 
