THE MICROBE OF PLEURO-PNEUMONIA. 
387 
No. 4. Normandy Cow, 18 months (actinomycosis), inoculated Dec. 
II, 1896, with 10 drops of pulmonary serosity. 
placed in the peritoneum of a rabbit, the other in that of a guinea 
pig. April 4th, both animals are killed : the bag of the guinea 
pig is full of transparent limpid liquid ; in the rabbit, very lean, 
the bag is found very flask and containing an opal fluid where 
the ordinary refringent small bodies are swarming. With the 
culture from the rabbit, bouillon is inoculated and dilutions to 
the I—100 and the i—1000 are put in two bags which are inserted 
in the peritoneum of two new rabbits. April 28th, both rabbits 
are killed ; culture has taken place in both bags, identical to the 
preceding one and very rich. April 29th, 10 drops of the dilu¬ 
tion to the i-iooo are injected under the skin, back of the left 
shoulder, to a Breton cow, 8 years old (cow No. 5). 
Here is the resume of the observation: 
Up to May 24th, everything remains normal ; no fever, no 
local lesion. 
May 25th (twenty-seven days after the inoculation), a tumor 
appears, as big as a Mandarin orange, hard, painful, at the point 
of inoculation ; this tumor spreads little by little May 31st, it 
is as big as a soup plate, extends under the shoulder, stiffens the 
movements of the animal, remains always very painful, the cow 
