THE MICROBE OF PLEURO-PNEUMONIA. 
389 
strikes a,t the hand that touches it. From this date the swelling" 
diminishes rapidly. June 6th all has disappeared. 
Reinociilated Oct. 7, ^^ 97 ? with one cubic centimeter of 
pleuro-pneumonic serosity collected on the 3d from a lung, seat 
of an acute lesion 5 this cow has presented no fever, nor local 
lesion at the point of inoculation. She was certainly vacci¬ 
nated by her treatment in the month of May. 
Fourth Series of Experiments.— January 19, 1898, a 
lung with lesions of acute pleuro-pneumonia, allows the collec¬ 
tion of several glass pipettes of pure limpid serosity from the 
sub-pleural lymphatic sacs. After having, by inoculation on 
agar and in bouillon, tested the serosity to be sure that it was 
free from ordinary bacteria, bags of collodion and of reed cane 
were prepared and filled with a dilution to 1-200. On Jan. 29th 
these bags were inserted in the peritoneum of two rabbits and of 
two guinea pigs. Each subject received one bag of each kind. 
February loth the four animals were killed. 
The bags of the guinea pigs have given no culture ; they 
contain a limpid and transparent liquid. 
On the contrary, the bags of the rabbits have all cultivated ; 
the liquid that they contain is cloudy, opal, free of the motile 
and refringent small points. In the collodion bags the culture 
is less abundant, it is very rich in the reed cane collodion ; the- 
liquid is milky. 
February iith at 9 A. m. a cow (No. 6 ) is inoculated under 
the skin, back of the left shoulder, with five drops of reed cane, 
bag culture, diluted in 2 c.c. of sterilized bouillon. 
Up to the 22d nothing abnormal; on that day there is at the 
point of inoculation a little sensibility on pressure, nothing else 5 
the temperature is 38.5°. On the 23d, swelling a little hot and 
painful, as big as the palm of the hand. The temperature goes 
above 39°. On the 25th there is a hard swelling, tense, warm, 
very painful, the size of a plate ; the following days, the swell¬ 
ing engages under the shoulder, the animal resists all manipu¬ 
lations ; the humor remains stationary to March 2d, then grad¬ 
ually and slowly diminishes and disappears. 
