654 
J. M. WRIGHT. 
reaction, either by causing an elevation of temperature or any 
systemic disturbance, ^he use of mallein as an agent in ascer¬ 
taining the animals which contain the bacilli cannot be extolled 
too highly. I would recommend its use in all doubtful cases 
and on all horses, mules or asses that have been exposed to 
glanders. 
To use mallein intelligently one must, in the first place, be 
sure that he has a good quality of the agent, and in making the 
test I would recommend the following method : 
The first mallein test should not be given earlier than ten 
days after the last glandered animal has been destroyed, nor 
later than three weeks, and the premises in the meantime should 
be thoroughly cleaned and disinfected. I have observed if the 
test be given immediately after the last glandered horse has 
been destroyed, that some will not react, but will do so later at 
a second test. Such a thing will rarely occur if two weeks are 
allowed to elapse after the last glandered animal is destroyed 
before giving the test. The test should cover a period of two 
days, and the first day the temperatures of the animals should be 
taken at 6 A. M., again at 12 m. and again at 6 p. m., to ascer- 
tain their normal temperatures. At q p. m. proceed to give the 
injection wfth a hypodermic syringe in the following manner: 
First thoroughly sterilize the skin on the cervical region be¬ 
neath the mane, with a strong antiseptic. Then burn the hypo¬ 
dermic needle in the flame of an alcohol lamp, load it with 
c.c. of mallein and inject beneath the skin at the point indi¬ 
cated in the cervical region. Great care should be exercised in 
using the hypodermic needle. It should be used but for one 
animal, or be thoroughly sterilized in the flame of an alcohol 
lamp before each injection, as neglect in this particular may 
result in the transmission of the disease from one animal to 
another. 
On the second day the temperature should be taken at 6 
A. M., 9 A. M., 12 M., 3 p. M., 6 p. M., and at 9 p. m., which 
completes the test. Great care should be exercised in taking 
the temperature from 6 A. m. to 9 p. m., for the simple reason 
