742 
PROF. LIGNIERES. 
has not the same influence upon nervous centres as that of para¬ 
plegic horses. This last seems to have the rather special prop¬ 
erty of acting upon the spinal cord. 
During my researches, I have made eleven post-mortems of 
paraplegic horses, received from all over. Out of these, I have 
found the streptococcus seven times in the subarachnoid fluid 
and twice or three times in the kidneys. In the other cases the 
tissues were already invaded with several microbian species. 
So as to avoid possible errors, I must say, that quite fre¬ 
quently a large streptococcus, not pathogenous, is found in the 
subarachnoid fluid ; this takes the Gram, is formed of plates 
flattened one against the other and growing vigorously in all 
medias of cultures, which differentiate it from the preceding. 
The results of these researches would be of greater value if 
the experiments of inoculation had been more frequent ; it is 
principally with those that I ought to have completed my 
studies. However, I believe that other inquirers may be as¬ 
sisted by knowing the means of cultures, the methods to follow 
to And the streptococcus and also the characters which may help 
to differentiate the streptococci of the pyogenes from those of 
the strangles type. 
As media of culture I prefer the following : Asparagus bouil¬ 
lon, loo parts, peptoned gelatine, 3,—to which may be added 
serum, 20 to 30. With this bouillon I have sometimes obtained 
magnificent cultures in eight or nine hours; the virulency is 
well conserved in it. 
To isolate the streptococcus, one may use with success intra¬ 
muscular injections to the rabbit. The microbe is specially 
found in the subarachnoid fluid. 
Characters by which the streptococci of the pyogenes type caii be differentiated 
frorn those of strongtes.* 
Streptococcus Pyogenes. Streptococcus of Strangles. 
The injection under the skin of the ear The same injection is most ordinarily 
produces quite frequently an enormous ery- unable to produce a typical erysipelas, 
sipelatous swelling. 
* These are not positive, they belong specially to streptococci obtained recently from 
the organism a great deal more than to those of cultures more or less modified by 
successive passages. 
