Bacteriological Studies of the Fixation of Nitrogen. 
35 
Solution lacking Sodium Nitrate (Na Noz.) 
Distilled water - - - 1000.00 c. c. 
Ca S0 4 - - - - - 9.4457 grams 
Na, S0 4 - - - - - 9.0668 grams 
Na, C0 3 - - - - - - - 2.8589 grams 
Na Cl - - - - - 20.2621 grams 
Iv 2 S0 4 - - - 2.0118 grams 
Mg SO 4 - - - 1.7475 grams 
Mannite - - 15.0000 graxns 
Solution lacking Sodium Sulphate (Na^SOO 
Distilled water 
Ca SO 4 - - 
Na, C0 3 - - 
Na Cl - 
Na NO s 
K, S0 4 - 
Mg SO4 
Mannite - 
Solution lacking Magnesium 
Distilled water 
Ca S0 4 
Na, S0 4 
Na, C0 3 
Na Cl 
Na NO s 
K 2 so 4 - 
Mannite - 
Solution lacking Potassium 
Distilled water 
Ca S0 4 
Na, S0 4 
Na, C0 3 
Na Cl - 
Na N0 3 
Mg SO4 
Mannite - 
1000.00 c. c. 
9.4457 grams 
2.8589 grams 
- 20.2621 grams 
13.5319 grams 
- - 2.0118 grams 
1.7475 grams 
- 15.0000 grams 
Sulphate (Mg SO*) 
1000.00 c. c. 
- - 9.4457 grams 
J 9.0668 grams 
2.8589 grams 
20.2621 grams 
13.5319 grams 
- - 2.0118 grams 
- 15.0000 grams 
Sulphate (K2SO+) 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
2.8589 grams 
- 20.2621 grams 
13.5319 grams 
1.7475 grams 
- 15.0000 grams 
Solution lacking Mannite 
Distilled water 
Ca S0 4 
Na, S0 4 
Na, C0 3 
Na Cl 
Na N0 3 
K, SO* - 
Mg SO4 
(C*H« 0 *) 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
2.8589 grams 
20.2621 grams 
13.5319 grams 
- -r 2.0118 grams 
1.7475 grams 
The chemical reaction of these solutions was left unchanged. 
Agars of four different strengths were prepared from each solution 
by diluting the solution once, twice and three times with an equal 
volume of distilled water. The resulting agars, then, contained the 
