34 
Colorado Experiment Station. 
used as a basis for' preparing the different agars, together with the 
composition of the various solutions from which the agars were made 
is given below. The solid medium was prepared by adding 15 grams 
of agar to each 1000 c. c. of solution. 
*Water soluble salts in soil used as a basis for synthetic agars. 
Ca SO, 
Mg S0 4 - - - - - 
k 2 so 4 ------ - 
Na, S0 4 ------ 
Na,CO, ------- 
Na Cl - - - - 
Na NO, ------ 
Silicic Acid - - 
Loss (water, organic matter, etc.) - 
The water soluble amounted to 2.97 per cent of 
material. 
Solution lacking Calcium Sulphate (Ca SO*) 
Distilled water - - - - 1000.00 
Na, SO, ------ 9 0668 grams 
Na 2 CO, ----- 2.8589 grams 
Na Cl - - - - 20.2621 grams 
Na NO, ----- 13.5319 grams 
IO S0 4 ----- 2.0118 grams 
Mg S0 4 ----- 1.7475 grams 
Mannite - - - - 15.0000 grams 
Cent 
15.902 
2.942 
3.387 
15.264 
4.813 
34.145 
22.781 
.252 
.471 
the air dried 
c. c. 
Solution lacking Sodium Carbonate (Na 2 CO2) 
Distilled water 
Ca S0 4 
Na, S0 4 
Na Cl 
Na N0 3 - - 
K, S0 4 - 
Mg S0 4 
Mannite 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
20.2621 grams 
13.5319 grams 
- 2.0118 grams 
- 1.7475 grams 
15.0000 grams 
Solution lacking Sodium Chloride (NaCl.) 
Distilled water ----- 1000.00 c. c. 
Ca S0 4 ------ 9.4457 grams 
Na, S0 4 ------ 9.0668 grams 
Na, CO, - - - - - 2.8589 grams 
Na N0 3 - - - - - - - 13.5319 grams 
K, S0 4 - - - - _ _ . 2.0118 grams 
Mg S0 4 - - - - - - - 1.7475 grams 
Mannite - - - 15.0000 grams 
*Eurnished by Dr. Headden. Bui. 155, p. 17, Analysis XV, Colo. Exp. Sta. 
