38 
Colorado Experiment Station 
also primarily asparagin and glutamin may arise, break down further into 
ammonia and form this, in presence of suitable carbohydrates—perhaps 
glucose—asparagin and glutamin are constructed. These amids would be 
thus, not the main products of decomposition, but rather the first stages in 
a higher synthesis and their formation from this point of view is not incon¬ 
ceivable. According to Hausteen’s experiments the amino-acids appear— 
so far as they have been investigated—much less adapted to the formation 
of proteids than ammonia and the two amids above mentioned. * ❖ * * 
This hypothesis of Schultze’s which has been recently supported by Balicka- 
Iwanoska (1903), (and also by Prianischnikow, 1904 and earlier), appears to 
us to explain best the tacts known to us at the present time in this difficult 
region of investigation, * * * 
‘ A new difficu-ty presents itself when we attempt to investigate how 
proteids arise from amino-acids and amids. It cannot be doubted that the 
process takes place in light and Pfeffer (1873) has further given proof that 
light acts indirectly in the process.” 
The nitrogenous material out of wliicli the proteids are built is 
primarily nitric acid, the principal intermediate products are am¬ 
monia and the amids, and these may occur in our wheat samples in 
va-rying quantities according as their transformation into proteid 
is more or less complete. We observe that corresponding to this the¬ 
ory we find the nitric, ammonic. amid, proteid and also the total ni¬ 
trogen highest in those samples grown with the application of ni¬ 
trates, which we used in liberal quantities for the purpose of dis¬ 
covering if possible the specific effects of nitric nitrogen, especially 
upon the character of the kernels produced. 
THK ASH CONSTITUENTS OF THE PLANTS 
We have not repeated the work of 1913 on this subject except 
to determine the ash constituents in one set of samples of the whole 
plant taken 5 August, 1915. We gave one set of samples for Fife 
in Part I, taken 31 July. The plants at this date in 1913 were as 
mature, or perhaps rather more so, than plants of the same variety 
on 5 August, 1915. The results obtained with the 1913 samples pre¬ 
sent the effects of the fertilizers upon the ash constituents under 
favorable seasonal conditions. The results of 1915 will serve to show 
the same thing under unfavorable seasonal conditions, and in these 
particular cases also the character and extent of the changes that 
we may attribute to the weather conditions. 
ASH CONSTITUENTS OF WHEAT PLANTS—CROP 1915 
Red Fife 
Ca. 
Mg. 
Fe. 
Mn. 
K. 
Na. 
P. 
s. 
Fertilizer per Acre 
Perct. 
Perct. 
Perct. 
Perct. 
Perct. 
Perct. 
Perct. 
Perct. 
Nitrogen, 80 pounds 
0.150 
0.107 
0.022 
0.004 
1.370 
0.020 
0.084 
1.409 
Phosphorus, 40 pounds 
.110 
.094 
.007 
.004 
.808 
.035 
.058 
1.527 
Potassium, 150 pounds 
.087 
.0100 
.012 
.004 
.980 
.031 
.001 
1.027 
None 
.105 
.073 
.014 
.004 
.972 
.041 
.091 
1.828 
The sulphur and chlorine were not determined. These determi¬ 
nations were omitted also in the 1913 series. 
