lO 
Colorado Experiment Station 
glistening, raised, structure appears flocculent under hand lens, 
gyrose under low power, margin undulating, general outline of 
colony round. Plates pure. Two colonies picked up on agar 
slants. 
A^o. 2 . Petiole from Stem No. i. —Petiole taken from part of 
stem where disease was advanced, tissue dark and watery; section 
0.5 mm. long, near juncture with stem, removed for plating. 
Colonies visible in 60 hours; same as those obtained from No. i, 
but first two plates contaminated, third plate pure. Made subcul¬ 
tures from two typical colonies. 
No. j. Petiole from Stem No. i .—Petiole taken from part 
of stem where disease was just appearing; tissue watery and olive- 
green in color. Colonies appeared in 60 hours; same as obtained 
from No. i; culture pure in second and third plates. Made cul¬ 
tures from two typical colonies on nutrient agar. 
No. 4 . Leaf ^ Blade from Petiole No. j.—Material used in 
plating taken from dark, watery part of leaf blade next to mid¬ 
rib; leaf well up on plant and clean. Colonies appeared in 60 
hours; same as preceding; pure in second and third plates. Picked 
up two characteristic colonies. 
No. 5 . Recent infection; tissue light olive-green and 
watery. Colonies appeared in 60 hours; same as in preceding 
isolations; plates pure. Picked up two typical colonies on agar 
slants. 
INOCULATION AND REISOLATION EXPERIMENTS 
PEAS 
September 21 , ip/ 5 .—Four varieties of peas were used at this 
time: Warshauer, Horsford, Alaska and Wellington. The plants 
were grown in 5 -ihch pots, one variety to a pot, and were fourteen 
days old when inoculated. 
A 24 -hour agar culture of the organism that was isolated from 
Stem No. i was used in this series; the agar growth was worked up 
in the water of condensation at the bottom of the slant, and the 
suspension thus obtained was employed as the inoculum. The 
stems to be inoculated were first scarified lightly with a sterilized 
scalpel until the surface was slightly moist, and then several loop- 
fulls of the culture were spread over the prepared spot, i to 2 centi¬ 
meters in length. Following this, the culture was pricked into the 
tissue with a sterilized needle. Three to four scarifications, 2 to 
3 centimeters apart, Avere usually made on each plant, and one 
plant thus treated was left in each pot uninoculated for a check. 
They were sprayed immediately after infection with sterile, distilled 
