12 
COI.ORADO Experiment Station 
long from the ground up; the others show the trouble just in the 
immediate region of the scar. In two of them, the infection has 
spread from the stem into the stipules, giving them a watery ap¬ 
pearance which has become yellowish brown in the older lesions. 
The frost has shrivelled all the vines except the check. Bacteria 
abundant in affected tissue. 
Wellington plants .—Four plants were inoculated with 'fourteen 
scarifications in all. As mentioned above, the frost seems not to 
have injured them appreciably. Almost no discoloration of the 
stems has occurred beyond the edge of the scarifications, and the 
vitality and general appearance of the plants are good. A little 
shrivelling has taken place where the inoculation was made, but 
there is no indication that the infection has spread. Bacteria are 
still present at the edges of the old lesions where the tissue appears 
darker and more watery, but none can be found in the scar proper. 
Apparently immune. 
Inoculations oe October 19 , 1915 —Peas 
The plants of this series were inoculated by pricking (cluster 
of stabs) the culture into the stem with a sterilized needle without 
first scarifying the surface. A 24 -hour agar culture of the organism 
isolated from Stem No. i was used. All plants were sprayed with 
sterile distilled water for four days after inoculation, and kept 
covered with bell jars for three days. 
Oh sensations 
Warshauer .—Five plants inoculated, and one check. After 
four days stems begin tO' have watery appearance at point of infec¬ 
tion; check normal in color. After twenty days, stems dark olive- 
brown and shrunken, leaves withered and brown, practically dead : 
check normal. 
Horsford .—Four plants inoculated midway of the vine toward 
the growing tip; one uninoculated check. After four days, sunken 
watery areas appear around the needle pricks; at the end of eight 
days, two of the plants are so badly shrunken and collapsed at 
points of inoculation as tO' allow the whole of the tips to droop. A 
microscopic examination of the watery tissue taken i cm. from 
needle prick shows the cells tO' be gorged with motile bacteria. The 
two remaining plants are watery and discolored, but have not col¬ 
lapsed. Check shows nothing of this shrivelling or discoloration. 
Causal organism reisolated from one of the two worst affected 
plants, q. v. page 18 . 
Inoculations oe November 25 , 1915 .— Horseord Peas 
All of the inoculations heretofore were made with one of the 
cultures isolated from Stem No. i in June. In order to determine 
