16 Colorado Experiment Station 
olive-brown in color thruout their entire length; the fourth plant is 
reacting positively, but slowly. 
Two of the plants inoculated with Culture 8 are still erect, 
but one is turning yellow and wilting, as described above; the 
stems of all the infected shoots are dark olive-brown in color. 
Feb. ip, ipi6. Culture 8 .—One plant dead; one plant with 
inoculated shoot dead and two new shoots; one plant with inocu¬ 
lated shoot dark olive-brown and two new shoots. 
Culture j.—The inoculated shoots of all four plants dead. 
Three plants have two new shoots, and one has one new shoot. 
Inoculations oe February 19, 1916.—Horsford Peas 
Thirteen vigorously growing pea shoots about to blossom 
were inocluated with a 24 -hour agar culture of Culture 3 by a line 
of single needle pricks; a fourth plant was pricked with a sterile 
needle as a control. The shoots were sprayed with sterile, dis¬ 
tilled water and kept covered with a bell jar for 36 hours. When 
examined at the end of this time, watery zones were visible around 
each needle prick for more than i mm., while the check showed 
nothing but the hole left by the puncture. 
Observations 
After four days the watery areas surrounding adjacent punc¬ 
tures had coalesced to such an extent as to produce a solid infec¬ 
tion over the whole stem. Control normal. 
March 8, ipi6 .—One whole stem dark olive-green, and infec¬ 
tion has spread over entire shoot; the disease has not spread as 
rapidly over second shoot, due possibly to the fact that the inocula¬ 
tion was in older tissue; third vine was accidentally broken off. 
Check normal. 
March 31 , ipi6 .—One plant, which was the less affected, has 
a black stem for 12 centimeters above ground, but it does not 
appear to be suffering particularly fromi the disease; it is growing, 
blossoming and seems vigorous. The inoculation in this case was 
on the lower part of the stem in rather old, hard tissue, and as has 
been obseiwed repeatedly, infections in the older tissue do not 
have the same disastrous effects as when they are made in the 
younger. The second plant was inoculated a little higher up, and 
while in practically the same condition as the other, it is not as 
vigorous. 
Inoculations of March 9 , 1916.—Horsford Peas 
To make certain that our stock culture of Culture 3 was re¬ 
maining pure, it was replated March 4 , 1916 , and a new culture 
picked up from agar colony March 8 . 
