A St^m Bught of FifIvD and Garden Peas 17 
In order to verify the new culture, it was inoculated from a 
24-hour agar streak into two pea vines that were in blossom. One 
shoot was pricked with a sterile needle for a check. All plants 
were in the same pot. They were sprayed as usual and covered 
with bell jar and newspaper for 48 hours. Placed in greenhouse. 
Observations 
March /j, ip/d.—All inoculations are taking; sunken watery 
areas, 3 mm. in diameter, around each needle prick, and where 
these are close together the lesions have coalesced, making a con¬ 
tinuous infection the entire length of the stem.- 
March ji, ipi6. —Inoculated shoots dark olive-brown, watery, 
and shrivelled; discoloration and watery appearance extends from 
stems along mid-ribs of stipules, causing a partial wilting of the 
latter. Dark, watery spots also present on leaves and stipules, 
which suggest stomatal infection probably resulting from washing 
germs from inoculated stems onto leaves while watering. Both 
infected vines have a sickly, pale-yellow color and are partially 
wilted. The check plant shows infection Jn two spots where the 
culture has apparently dripped during watering. 
April 14, 1914. —All shoots brown and dead, including check, 
from lowest point of inoculation out tO' the growing tip; lower 
part of vines green. 
Inoculations of AIarcii 23, 1914 
The purpose of this series was tO' determine whether the 
organism which is responsible for the pea disease is likewise 
pathogenic for other common legumes. Twenty-four hour agar 
cultures were used in all of the infections. In addition to needle 
prick inoculations in the stems and leaves, the entire plants were 
sprayed with a suspension of the culture in physiological salt solu¬ 
tion. Just previous to this operation, they were thoroly sprayed 
with distilled water and then with the culture, until the liquid 
dripped from the leaves. Our object in spraying with the culture 
was to determine whether the stomata might not serve as a channel 
of infection, as well as mechanical injuries. All plants were 
sprayed with distilled water at frequent intervals and kept covered 
with bell jars for 48 hours after inoculations. 
Horsford Garden Pea. —After four days, characteristic 
watery, olive-green depressions appeared around the needle pricks. 
After six days, splendid stomatal infections, appearing as dark, 
watery, green spots, developed on the leaves which had not been 
subjected to mechanical injury. (Plate II, Fig. i.) 
Golden Wa^ Bean. —After four days, there appeared around 
the needle pricks on the bean pods, grayish brown patches, .95 mm. 
