i8 Cox/)RADO Experiment Station 
in diameter. Similar injury with a sterilized needle did not pro¬ 
duce the same results. Inoculated stems and leaves were 
unchanged. ; y'i^ 
April 14, 1916.—The spots on the bean pod remained un¬ 
changed and there was no evidence of the disease in any other 
parts of the plants. 
Alfalfa .—April 14, 1916.—The plants appeared to be entirely 
immune to the disease. 
Sweet Clover .—April 14, 1916.—Entirely immune. 
Crimson Clover .—April 14, 1916.—Entirely immune. 
Mammoth Clover .—April 14, 1916.—Entirely immune. 
Cow Pea .—April 14, 1916.—Entirely immune. 
REISOLATION OF CAUSAL ORGANISMS FROM PLANTS 
INOCULATED WITH PURE CULTURES 
First Reisolation, October 2"/, 1^15 
Platings in nutrient agar were made from sections taken from 
pea stems which had been inoculated October 19 with a pure cul¬ 
ture from Stem No. i and which had developed the blight with all 
the typical symptoms. The tissue used came from two different 
parts of the vine where it appeared watery and olive-green in color 
some distance from the point of infection. No' attempt was made 
to sterilize the surface of the stems from which this was taken, 
previous to plating. The plates gave a pure culture, and from the 
resulting colonies, eighteen were picked up on agar slants; ten of 
these were selected and reinoculated intO' Warshauer field peas 
November 15. Typical blight resulted in every case. 
Second Reisolation, December 7. 1915 
The plant from which the reisolation was made was inoculated 
November 25, 1915, with one of the cultures originally isolated 
from an infected leaf blade, June 4, 1915. 
Two sets of plates were made fromi this plant, one from the 
stem and one from the base of a watery petiole where the disease 
was just starting. The stem was yellow, watery and sunken over 
the whole area of needle pricks, not only around them, but between, 
as well. 
In all former isolations, no precautions were taken to sterilize 
the outside of the stems previous to plating out the tissue. How¬ 
ever, in the present case it was wiped with alcoholic mercuric 
