20 
Colorado Experiment Station 
2. ENDOSPORES. —No spores have been observed. Agar cul¬ 
tures 6 days and 48 days old were stained for spores both with 
Moller’s stain and with Zeihl-Nielson carbol fuchsin, with negative 
results. 
3. ELAGELI.A. —The organism is motile by means of a single 
polar flagellum readily demonstrable in 18-hour agar cultures 
stained by Loeffler’s method. According to Migula’s classification, 
this places the organism in the genus, Pseudomona's. 
4. CAPSULES.' —No capsules could be demonstrated either by 
Welch’s method or by that of Hiss. 
5. zooGLOEA.— No' zO'Ogloea have been observed. 
6. INVOLUTION FORMS. —None observed. 
7. staining REACTIONS. —The germ stains readily with all of 
the ordinary aqueous stains. Both aqueous and carbol fuchsin 
bring out the coarsely granular protoplasmi of the cell, together 
with what appear to be polar bodies. Loeffler’s methylene blue 
shows nothing unusual altho at times there is a suggestion of ir¬ 
regular staining. Neisser’s stain reveals neither polar bodies nor 
any special intracellular structures. It is Gram negative and is not 
acid fast. Neither starch nor glycogen were demonstrated. Both 
anilin-water genitan violet and anilin water fuchsin stain it readily. 
II. CULTURAL FEATURES 
1. AGAR stroke.— Growth moderate in 24 hours; abundant in 
4 days. Form of grozifth —filiform, margins irregular. Elevation 
—flat. Lustre —glistening. Topography —smooth. Optical char¬ 
acters —translucent. Chromogenesis'^ —grayish white, Engine Color 
No. 13. Odor —decided, rotten cabbage. Consistency —^butyrous. 
Medium —slightly greened. 
2. POTATO. — Grozuth —moderate, persistent. Form of growth 
—filiform. Hlez^ation of grozuth —slightly raised. Topography — 
smooth. Odor —absent. Chromogenesis —cream, orange yellow 
tint No. 2 in three days. Engine color No. 7 in five days (light 
orange). Consistency —waxy and slightly viscid. Medium —gray- 
brown in fourteen days. 
3. loeffler’s BLOOD SERUM. — Stroke —moderate in 72 hours. 
Form —filiform. Elezfation —slightly convex. Lustre —^^glistening. 
Topography —smooth. Chromogenesis —pale yellow. Engine color 
3a. No liquefaction. Medium unchanged. 
*Color terras refer to Standard colored papers made by Milton Bradley 
Co., Springfield, Mass. 
