22 
COI.ORADO Experiment Station 
below, and is two-thirds blued after ten days. Following this, the 
litmus is reduced and the whole tube assumes a gray color in fifteen 
days. At the end of twenty days the peptonization is complete and 
the fluid is dark blue to dark blue green in color. Coagulation 
begins on the fifth day, followed by clearing, which is complete in 
twenty days. 
20° C.—Milk begins to turn blue from the surface downward 
after seven days, becoming lighter below, and is completely blued 
after fourteen days. Following this the fluid becomes gray with a 
blue ring at the surface, and at the end of 35 days it is between 
dark blue gray and dark green gray, i. e. green gray No. 2 and 
A-blue green dark, in color. Coagulation begins the seventh day, 
followed by peptonization, which is complete in 35 days. 
10. GELATIN COLONIES. — Growth —moderately rapid at 20° 
C. Form —round. Blevation —crateriform, liquefying. Edge — 
entire. Liquefaction —saucer. Size —4 mm. diameter in three days. 
11. AGAR COLONIES. — Grozvth —moderately rapid at 20° C. 
Form —round. (Plate I, Figs. 3, 4.) Blevation —slightly convex. 
Edge —undulate. Internal structure —gyrose, marked by wavy 
lines indefinitely placed. (Fig. III.) Size of majority —2 mm. in 
five days. Deep colonies lens-shaped. (Plate I, Fig. 5.) 
FIG. Ill 
12. starch jelly.— Prepared from Uschinsky’s solution and 
potato starch. Grozvth —moderate. Medium —stained yellowish 
srreen. Diastatic action —absent. 
13. corn's SOLUTION.— No growth. 
14. uschinsky's solution. —No growth. 
15. EERMi's SOLUTION. —Growth abundant, heavy clouding, 
white wrinkled pellicle at surface in ten days and small amount of 
sediment. 
