A Stem Blight oe Field and Garden Peas 23 
16. NAEGLi’s SOLUTION. —Slight transient clouding. 
17. fraenkel's solution.— Heavy clouding and surface 
scum in ten days. 
18. dunham^’s solution. —Uniform', heavy clouding and con¬ 
siderable white compact sediment, somewhat viscid on agitation. 
19. ASPARAGiN SOLUTION.— Growth, abundant. Fluid, col- - 
ored light green. 
20. sohngen's SOLUTION.— (Urea.) No growth. 
21. SODIUM CHLORIDE IN BOUILLON. —Nutrient broth contain¬ 
ing 1% peptone, but without salt, was used in this determination. 
To measured quantities of this medium, different amounts of 
chemically pure sodium chloride were added, giving a range in the 
salt content from 0.0% to 5.0%. Four tubes of each concentra¬ 
tion were prepared, and each set differed from the others by 0.25%. 
One loop of a 24-hour broth culture was used for the inoculation, 
and the tubes were kept at 28° C. 
Growth occurred in 24 hours in all concentrations up to 2.0%. 
No difference could be observed in the amount of growth from 0.0% 
to 0.75%, however, with 1.0% and 1.25% there was less, and 
with 1.5% and 1.75% there was still less. After 48 hours, growth 
appeared in the tubes containing 2.0'% to 2.75%; after three days 
in 3.0% and 3.25%; after eight days one of the set with 3.5% 
became turbid. Beyond this point no growth took place, 3.75% 
being sufficient to prevent growth. 
The detailed results of this experiment are given in Table I. 
