24 
Colorado Experiment Station 
TABLE I 
The Effect of Sodium Chloride upon Growth in Bouillon 
%NaCl 
Days Before 
Growth Appeared 
• 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
11 
12 
13 
14 
15 
0.00 
+ 
+ 
+ 
+ 
+ 
4- 
4- 
4- 
4 - 
4 - 
4 - 
4- 
4" 
4- 
4- 
.50 
+ 
+ 
+ 
+ 
+ 
4- 
4- 
4- 
4- 
+ 
4- 
4- 
4- 
4- 
4- 
.75 
+ 
+ 
+ 
+ 
+ 
4- 
4- 
4- 
4- 
4- 
4 " 
4* 
4- 
4- 
4- 
1.00 
+ 
+ 
+ 
4- 
4- 
4- 
4- 
+ 
4“ 
4- 
4- 
4- 
4- 
1.25 
+ 
+ 
+ 
+ 
4- 
4- 
4- 
4- 
4- 
4- 
4- 
4~ 
4- 
4- 
1.50 
+ 
4 - 
+ 
4- 
+ 
4- 
4- 
4- 
4- 
4- 
4- 
4- 
4" 
4- 
4- 
1.75 
+ 
+ 
+ 
+ 
+ 
4- 
4~ 
4- 
4* 
4- 
4- 
4 - 
4- 
4- 
4- 
2.00 
— 
+ 
+ 
+ 
+ 
4 - 
4- 
4" 
4- 
4- 
4- 
+ 
4 - 
4- 
4- 
2.25 
— 
-f 
+ 
+ 
+ 
4- 
4- 
4- 
4" 
4- 
4- 
+ 
4- 
4- 
4- 
2.50 
— 
+ 
+ 
+ 
4- 
4- 
+ 
4- 
4- 
4- 
4- 
4 " 
4- 
4 - 
4- 
2.75 
— 
+ 
+ 
4“ 
4- 
4 - 
4 - 
4- 
4- 
4 - 
-i- 
4- 
4- 
4 
3.00 
— 
— 
+ 
4- 
4- 
4 - 
+ 
4- 
4- 
4- 
4 “ 
4* 
4 - 
4- 
3.25 
— 
— 
+ 
4“ 
4- 
4 - 
+ 
4 - 
4 - 
4- 
4- 
4- 
4- 
4- 
3.50 
— 
4 - 
+ 
4 - 
4 - 
4 - 
4- 
4" 
4- 
3.75 
— 
4.00 
— 
— 
— 
4.25 
— 
— 
4.50 
— 
— 
— 
— 
4.75 
— 
— 
5.00 
— 
,, 
— 
22. GROWTH IN BOUILLON OVER CHLOROFORM. -To 10 C.C. 
of nutrient broth in test tubes, 5 c.c. of chloroform were added with 
aseptic precautions. Duplicate tubes thus prepared were inoculated 
with one loop of a 24-hour broth culture and held at 20° C. No 
growth took place in the tubes containing the chloroform after 60 
days, while a check tube of plain broth gave abundant growth in 24 
hours. 
23. NITROGEN. —Peptone, asparagin, urea and ammonium 
tartrate have been used in different media as a source of nitrogen 
for the organism. Nitrogen was obtained very readily, as would 
be expected, from the first of these, as it occurred in nutrient broth, 
nutrient gelatin, nutrient agar and Dunham’s solution. Good 
growth was also secured from the nitrogen of the asparagin present 
in Jordan’s asparagin solution. 
