A Stem Blight of Field and Garden Peas 
25 
Jordan’s A spar agin Solution 
Redistilled water.1000 c.c. 
Asparagin . 2 grams 
MgSO^. 1 'gram 
KgHPO^ . 1 gram 
No growth was obtained from either the urea nitrogen in 
Sohngen’s solution, or the ammonia nitrogen in tlie ammonium tar¬ 
trate of Cohn’s solution. 
Sohngen’s Solution 
Tap water .500. c.c. 
Urea . 25. grams 
K 2 HPO 4 .25 gram 
Calcium malate . 2.50 grams 
24. best media for long-continued growth. —Standard 
nutrient agar 
25. QUICK TESTS FOR DIFFERENTIAL PURPOSES. —Liquefaction 
of gelatin; coagulation and peptonization of plain milk; growth in 
Fermi’s solution, absence of growth in Uschinsky’s and Cohn’s solu¬ 
tions; production of acid from dextrose, galactose and saccharose 
in sugar free broth; yellowish-green color prodnced in starch jelly. 
III. PHYSICAL AND BIOCHEMICAL FEATURES 
1. GAS PRODUCTION. —The power of the organismi to produce 
gas from dextrose, galactose, saccharose, mannite, laevulose, mal¬ 
tose, inulin, lactose and glycerine was determined by adding i% of 
the different fermentable substances to +io sugar free bouillon. 
Two fermentation tubes of each were prepared, and after steriliza¬ 
tion, they were inoculated with a 72-hour agar culture and placed in 
an incubator at 28° C. N^o gas ims formed in any case. No 
growth occurred in the closed arm, and there was a sharp line of 
demarcation between the growth in the upper part of the U and 
the closed arm. After fourteen days the reaction of the different 
inoculated broths was tested with litmus, and it was found to be 
acid in the case of glucose, galactose and saccharose, and alkaline 
in the remainder. 
2. AMMONIA PRODUCTION. —Nutrient bouillon containing 1% 
peptone and Jordan’s asparagin solution with 0.2% asparagin were 
used in determining the power of the organism to produce ammonia. 
Duplicate 100 c.c. portions of each solution in 250 c.c. Erlenmeyer 
flasks were inoculated with twO' loops of a 24-hour broth culture and 
kept at 20° C. Uninoculated controls of each solution were carried 
along with these at the same time. After five days, both cultures 
and controls were analyzed for ammonia by distillation with mag- 
