8 
The Coeorado Experiment Station. 
solution of hydric chlorid in very different degrees. In those 
cases in which the amount of xylan was high, we found that boil¬ 
ing for thirty minutes did not suffice for the accomplishment of our 
purpose, i. e., to carry the action of the hydric chlorid to an end. 
§i6. In some fodders and dungs we found it necessary to boil 
them two, and in a few cases, even three times with separate por¬ 
tions of the acid in order to obtain results which agreed reasonably 
well. We found, too, that the inversion of this extract was much 
more difficult than of any of the others,—it being necessary to use 
ten c. c. cone, hydric chlorid and to digest in the water bath for two 
hours; this means at our altitude, a temperature of about 85° to 
possibly 90° C. in the solution, even though the water in the bath 
is kept boiling briskly. We found that this quantity of acid and 
time gave us the highest results, measured in terms of the reducing 
power, and usually a good agreement between the duplicates. 
§17. The sodic hydrate used was also a one per cent, solution. 
The reducing power of this extract, after inversion, was, as a rule, 
very small. We observed in this connection that the reducing 
power of this extract was frequently higher in those cases in which 
w^e found it difficult to complete the extraction with hydrochloric 
acid than in others. I interpret this variation as suggesting that 
the small amount of reducing sugars found in the sodic hydrate 
extract may belong to the hydric chlorid extract rather than indi¬ 
cate the presence of a hexose sugar. 
§18. The residue left after treatment with sodic hydrate was 
washed free from Na O H, dried, etc. It was next moistened with 
water and spread out on the inner surface of a small flask which was 
connected with a chlorin apparatus and allowed to stand for an hour 
after the flask had become entirely filled with the gas. It was then 
washed, boiled with dilute Na O H and subsequently with H2 S O3 
and the residue finally dried and weighed as cellulose. The action 
of chlorin, etc., did not produce any reducing sugars, at least there 
were none present in the filtrate. 
§19. In Bulletin 93, we have recorded our results in deter¬ 
mining the coefficients of digestion of the groups, substances usually 
taken cognizance of in our ordinary fodder analyses, i. e., Dry Mat¬ 
ter, Ash, Fat, Protein and Nitrogen-free Extract; in the present 
bulletin, I intend to present the results of these same experiments 
using a different series of groups, or to give the coefficients of di¬ 
gestion for the alcoholic extract, cold water extract, hot water ex¬ 
tract, one per cent, hydic chlorid extract, one per cent, sodic hydrate 
extract, chlorin extract, and lastly, of the cellulose or that portion 
which persistently resists the action of these reagents. In addition 
to the preceding coefficients, I shall give the coefficients of digestion 
