Colorado Fodders. 
19 
§49. I will anticipate a general result which will be evident, 
when the determinations of this group in all the samples have been 
given, i. e., that it is present in hays made from grasses and legumi¬ 
nous plants, also in corn fodder, sorghum and the saltbush, Atriplex 
argentea, being most abundant in the last named plant; further that 
the percentage of this group present in the dung is approximately 
twice that in the hay or fodder, except in the case of the saltbush in 
which it is only one and one-half times as much as in the saltbush 
itself. 
AMID NITROGEN IN AEEAEEA HAY. 
§50. In calculating the proteids we have multiplied the total 
nitrogen by 6.25 consequently our product is too high, as more or 
less of the nitrogen is present in the form of amids, compounds con¬ 
taining a higher percentage of nitrogen and, consequently, having a 
factor less than 6.25, if for example, the nitrogen were present as 
asparagine, its factor would be 4.7. 
§51. In the following statement I have given the proteid 
equivalent to the amids. I have made no attempt to divide the ni¬ 
trogen compounds further than into the two groups, proteids and 
amids. 
§52. The amid nitrogen found in samples of alfalfa hay cut 
at half bloom is as follows, 0.372, 0.350, 0.614; average is 0.444 
which is rather far removed from the maximum which is, however, 
the highest found in eleven samples. The amid nitrogen found in 
the alfalfa hay used in this series of experiments was 0.446 agree¬ 
ing very well with the average found for the other samples cut in 
half bloom. 
§53. The proteids in the alfalfa hay fed, corresponding to 
the amid nitrogen, amounted to 372.11 grams; in the orts 34.66 
grams; consumed 337.45 grams; voided 62.23 grams; digested 
275.22 grams, from which we obtain 81.55 for the coefficient of di¬ 
gestion for the amid nitrogen. 
THE PROTEIDS REMOVED BY THE VARIOUS SOLVENTS AND THEIR CO- 
EEEICIENT OE DIGESTION. 
§54. As the boiling 80 per cent alcohol removed practically 
all the coloring matters from the hays, I deemed it advisable to ex¬ 
tract them with anhydrous ether and determine the nitrogen re¬ 
moved in this manner. I have assumed that the nitrogen thus re¬ 
moved is principally contained in the coloring matters. For this 
extraction, I use 30 grams of air-dried hay, macerated it with 150 
c. c. of ether for 16 hours, and then subjected it to extraction in a 
soxhlet extractor for ten hours. Our air-dried hay carries from 5 
to 7 per cent, of moisture. I have repeatedly compared the results 
