98 
J. W. CONNAWAY. 
These experiments were not regarded as conclusive, since 
the season of the year at which the work was done, and the 
youthfulness of most of the animals inoculated, might account 
for the result. In order to give the matter a more decisive test 
a larger experiment was planned for the following summer, and 
carried out in conjunction with the Mississippi Experiment 
Station in June and July, 1897. A full report of the results 
upon the cattle shipped to the Mississippi Station appears in Bul¬ 
letin No. 42 of that Station. Only a summary is given here. 
Eleven head were inoculated, eight of these at the Missouri 
Station before shipping, and three after their arrival in Missis¬ 
sippi. These cattle varied in age from one and one-half years 
to eleven years. They were inoculated daily with serum in 
doses of 40 to 60 c.c., according to the size of the animal. The 
minimum quantity injected into any one animal was 146 c.c. ; 
the maximum quantity was 772 c.c. After tick-infestation, all 
of them suffered from acute attacks of the fever, and all except 
two of the younger animals (2 years old) died. 
During the same summer five head more were inoculated at 
the Missouri Station, two mature cows and three yearling 
steers. The material used in this experiment came from differ¬ 
ent sources than that used in the Mississippi experiment—one 
lot was obtained from the Texas Station, and another from a 
Texan animal at the North. One cow received subcutaneously 
a total of 230 c.c. ; the other one 420 c.c., in doses of 20 to 40 
c.c., over a period of two weeks or more, immediately preceding 
tick-infestation. Both died from acute attacks of the fever, fol¬ 
lowing tick-infestation. Not the least mitigation in severity of 
the attack appears to have resulted from the use of serum. 
The three yearling Jersey steers were inoculated with doses 
of 10 c.c. to 40 c.c., over a period of two weeks ; one steer received 
144 c.c., another 320 c.c., and the third 180 c.c. All of these 
suffered from attacks of the fever after tick-infestation, but re¬ 
covered. A check animal, not inoculated, appeared to suffer 
more severely. This animal, however, was somewhat less 
vigorous. 
