110 
J. W. CONN AW AY 
In addition to the tick infestation, all the calves were inoc¬ 
ulated with infected blood. June 15th, previous to the second 
period of infestation, 3 c.c. of blood from a Texas cow was in¬ 
jected subcutaneously into bulls Nos. 15, 16, 17 and 21 ; they 
were again inoculated on August 4th with 5 c.c. each from the 
same animal. 
No. 19 was inoculated June 13th with 4 c.c. from a native 
recovered cow, and six days later infested with several hundred 
ticks. June 29th, 16 days after the inoculation and 8 days after 
tick-infestation, the calf appeared to be in good health, the 
temperature was normal, and the percentage of blood cor¬ 
puscles from haematokrit readings was 36 per cent, (about 
normal). On July 6th, 23d day after inoculation and 15 days 
after tick-infestation, the percentage of corpuscles had fallen 
to 20 per cent. On July 8th, the hsematokrit reading was 13 
per cent., the morning temperature 104.9, all d evening temper¬ 
ature 106.5 F. ; ticks in second moult, animal off feed, sluggish 
and gaunt. This condition appears to have arisen from 
the tick-infestation rather than from the blood inoculation. No 
rise of fever was noted at the usual period after inoculation. 
This calf was a little dumpish all summer, but has fed well. 
None of the other calves showed any outward symptoms of 
illness apparent to the average observer; they were never off 
feed ; the thermometer, however, showed that all of them had 
some elevation of temperature during the season and there 
was considerable destruction of blood corpuscles, as determined 
by means of the hsematokrit. Bull No. 15 showed a diminu¬ 
tion of corpuscles from 37 to 18 per cent. ; No. 16, from 
44 to 20 per cent. ; No. 17 fell to 24 per cent. ; No. 21 showed 
but little diminution in percentage of corpuscles. The greatest 
fall in percentage appeared to result not from the inoculation 
and earlier tick-infestation, as it occurred later in the fall after 
the pastures had become dry and grass scanty, and ticks more 
numerous on the pasture. All these calves had about regained 
the normal percentage of corpuscles at close of the experi¬ 
ment. 
