330 
J. W. CONNAWAY. 
calves appeared normal. Their appetite improved, and their 
blood corpuscles increased in numbers. By the 25th day the 
average per cent, had risen to 31. 
The secondary fever period began on the 28th or 29th day. 
It greatly resembled the primary one in its severity, but differed 
from it in continuing longer, and in appearing in some animals 
on the 28th and 29th day, while in others it appeared on the 
34th or 35th day. The hsematokrit readings also show a second 
fall in the blood corpuscles. This reached its lowest average 
(21 per cent.) on the 39th day. The lowest individual on that 
day was 14 per cent., the highest 26 per cent. The secondary 
reaction must be regarded as due to the activity of the micro¬ 
organism in the blood, and not to a reinfection from without. 
Every calf was examined with great care for ticks until the 90th 
day, but none were found. Moreover, in the case of the Tod 
bulls inoculated the previous fall at the Missouri Station there 
was no possibility of the secondary reaction being due to a sec¬ 
ondary invasion of the micro-parasites from tick-infestation, as 
these bulls were in noninfected territory. The secondary fever 
period did not terminate so abruptly as the primary, but showed 
a tendency to an occasional high temperature. For instance, 
we found No. 3 with a temperature of 105° on the morning of 
the 47th day, and No. 1 with 103.5 0 on the 54th day, and No. 4 
with 103.8° on the 59th day. The calves gained in weight and 
growth, and their blood showed some improvement, which was 
slow but steady. 
Second inoculation .—2 cc. of defibrinated immune blood 
was given on the 77th day (March 23d) subcutaneously to all 
except No. 4. A mild reaction followed in about eleven days; 
the highest morning temperature found was 104.1°. Calf No. 4 
was not given the second dose of 2 cc. with the others, and 
showed a morning temperature of 104.2° on the 94th day, which 
was at the same time the other calves were reacting from the 
second inoculation. No record of blood changes was possible, 
because the luematokrit was out of order at that time. The 
first reexamination of the blood occurred on the 106th day 
