ANTISEPTIC THERAPEUTICS. 
739 
Psoas Muscle .—In all accounts of azoturia the psoas muscle 
is spoken of as exhibiting - a marked degree of fatty degenera¬ 
tion. In every case examined this muscle on section was nota¬ 
bly pale, and bits of it teased out in glacial acetic acid showed 
many fine and coarse fat drops, both free and within the muscle 
fibres. The same appearance was noted in a case of pneu¬ 
monia. 
Microscopic Anatomy — Technique .—All tissues were fixed 
in Zenker’s fluid for twenty-four hours, washed in running 
water overnight, and hardened in 80 per cent, alcohol. The 
material was then cut into small pieces and passed through two 
changes of 95 per cent, alcohol, two changes of absolute alco¬ 
hol, one of chloroform, and one of chloroform saturated with 
paraffin, remaining in each twenty-four hours. The tissue was 
then embedded in hard paraffin and cut. Sections from each 
organ were stained with Unna’s methylene blue and eosine, 
Mallory’s connective tissue stain, and alum hsemotoxylin. 
Liver .—All the livers examined had certain characteristics 
in common. The capillaries in all were markedly injected. 
The liver cells contained fine brown granules, and the perivas¬ 
cular spaces were dilated, containing granular material taking 
the acid stain, presumably precipitated albumen of the serum. 
This pigment in the liver cells occurs in the livers of normal 
horses, but not to the extent seen in azoturia. Aside from these 
slight variations from the normal, two distinct types of lesion 
were found. In five cases the picture was one of a diffuse pro¬ 
cess, while in four the lesion was distinctly local in character. 
In the first type the centres of the lobules seemed most 
affected. In the area around the central vein the liver cells 
were relatively small, distorted, and presented a greater or less 
degree of vacuolation. 
In the second type, small areas, bearing no constant relation 
to the anatomical structures of the organ, were to be seen, in 
which the liver cells had more or less completely disappeared, 
the space left being filled with dilated capillaries. Around such 
areas the nuclei of the liver cells frequently did not stain, the 
