GEORGE JOBSON. 
113 
Albumin.— Test No. 10. — Acidulate a quantity of urine with 
a few drops of acetic acid, then add a saturated solution of po¬ 
tassium ferrocyanide. This gives a precipitate when much 
albumin is present, and produces an opalescence when there 
is only a trace. -This test will show as little albumin as i part 
in 60,000. 
Test No. 20 .—Pour a small quantity of fuming nitric acid 
into a test tube and add a little warm urine in such a manner 
that the urine floats on top. If albumin is present a white zone 
is formed at the line of demarcation. If the urine is not warmed 
before making the test, urates may give a similar reaction. 
A great many substances besides albumin will react to any 
test which is used for this substance, as salol or also resins, in 
the urine. But on addition of an excess of pot. ferrocyanide 
these precipitates are redissolved. Before testing for albumin 
urine must be made perfectly clear by filtering, otherwise you 
will not be able to detect traces. 
Sugar.— Test No. / 1 .— Test Fehling’s solution, which is a 
mixture of copper sulphate, caustic soda and Rochelle salts, by 
boiling. If it remains clear it is in good condition. Take equal 
parts of urine and Fehling’s solution and raise to the boiling 
point. A yellowish or reddish precipitate stands for sugar. 
Test No 20 .—This is probably the most reliable test known 
for sugar. To 2 drams of urine add the point of a knife full of 
phenyl hydrazin chloride, and the same amount of sodium ace¬ 
tate crystals ; shake thoroughly until dissolved. Then place in 
a hot-water bath just below boiling point for 30 minutes. Re¬ 
move test tube and cool the solution under running cold water 
and examine some of the yellow precipitate with a microscope. 
If sugar be present yellow needle-like crystals are seen. Before 
testing for sugar if albumin is present, remove by acidulating 
with acetic acid, boil and filter. 
Blood.— To a little tr. guiac. add an excess of peroxide of 
hydrogen. To this add the suspected urine. A blue color 
stands for blood. 
Pus. —Acidulate a small portion of urine with acetic acid. 
