696 
VERANUS A. MOORE. 
Case No. j. (Cases 3 to 5 were in the second herd.) This was in a 
cow, seven years old. The right hind leg was affected. The disease had 
already run about three weeks and for several days the animal had been 
under the care of a veterinarian. The cellulitis had extended up the 
leg to and abcwe the hock joint and down to and over the coronary 
cushion. The hoof covering the heel had cracked and part of it had been 
removed. There was a large subcutaneous abscess above the heel, which 
was discharging through an opening or crack in the hoof near the mid¬ 
dle of the bottom of the foot. It was stated that at first the pus was 
thick and of a cream color, but at this time it was thin and of a dirty 
brown tint. By means of pressure a considerable quantity of it was 
forced out, from which tubes of agar were inoculated and a few cubic 
centimeters placed in a sterile tube and brought to the laboratory. 
Case No. 4. This was in a two-year-old heifer. The left hind foot 
was attacked. The leg was slightly swollen. There was distinct fluc¬ 
tuation over an area about 3 cm. in diameter, on the front of the foot 
just above the hoof. The animal seemed to be well otherwise. Tem¬ 
perature normal and appetite good. The hair was clipped, the foot care¬ 
fully washed and disinfected and the abscess opened. It contained about 
5 c.c. of a thick creamy looking pus. Several tubes of agar were 
inoculated from this and the remainder placed in sterile tubes for 
further examination. 
The results of the bacteriologic examination of the cases 
from which cultures were made are as follows : 
From Case No. 1.—A streptococcus and an undetermined 
micrococcus and a bacillus were obtained. 
From Case No. 3.—A streptococcus and several (about six) 
forms of chromogenic micrococci and bacilli. 
From Case No. 4.—A streptococcus which appeared for the 
greater part in pure culture. In three tubes B. coli communis 
was present. 
In the plate cultures in agar made with the pus from cases 
Nos. 1 and 4 colonies of the streptococcus predominated. The 
relation of the streptococcus to the disease was determined by 
the following inoculations. 
Two cows were inoculated with the purulent material ob¬ 
tained from cases Nos. 3 and 4, respectively. A small portion 
of the pus was diluted in sterile bouillon and about 2 c.c. of the 
suspension injected subcutaneously just above the hoof in the 
left fore foot in each animal. Swelling was noticed on the 
third day. It gradually extended up the leg to the knee joint. 
There was much tenderness and evidence of pain. The sub¬ 
cutis became indurated in the lower part of the leg, and on the 
