STREPTOCOCCI IN COMPARATIVE PATHOLOGY. 
785 
and Cadiac found streptococci in the lesions of animals suffering 
from this disease. Although differences appear to exist in the 
streptococci obtained by these investigators there is a striking 
similarity in the descriptions of these cultures. In the absence 
of fresh cultures of these organisms it is unwise to speculate 
upon their identity, or dissimilarities. It seems quite clear, 
however, that if any of these streptococci are demonstrated to 
be the cause of this disease the honor of its discovery belongs to 
Schiitz. 
Lignieres,* who had exceptionally good opportunities in 
1 895-96 for studying this disease found the streptococcus of 
Schiitz and identified it with the streptococcus of strangles. 
Lignieres, however, concluded from his investigations that the 
cause of the disease was a cocco-bacillus f which he isolated with 
* Loc. cit. 
f F01 the convenience of those who do not have Lignieres original article his de¬ 
scription of the organism and the method of obtaining it are appended. 
“ When one examines the preparation after it has been stained the bacteria present 
themselves under the form of very small diplococci, isolated cocci, and longer forms with 
rounded ends. These last cocco-bacilli represent the tube form of the microbe. At the 
moment of division they appear as diplococci, while the micrococci result from the com¬ 
pleted and recent separation of diplococci. 
The microbe also often appears as a diplococcus when it is examined without staining. 
In some special conditions the cocco-bacillus elongates and presents itself under the 
form of a genuine small bacillus, binally, in the pus of experimental abscesses obtained 
by subcutaneous injection in the horse, it takes the form of streptococci-bacilli. 
It does not form spores, and it is killed in less than a quarter of an hour at 65 °C.” 
Cultures .—“ It is an aerobic organism. Its first cultures are difficult to obtain, but 
when it has been accustomed to artificial media it grows rather abundantly. 
In peptonized bouillon, after twenty-four hours, one observes a uniform turbidity, 
which lasts for several days. The reaction of the medium does not change. 
Plain bouillon is not so favorable for growth. 
o 
On gelatine, at a temperature of 20 r C., it forms after two or three days rounded colo¬ 
nies which are at first transparent, and then slightly opaque and white, and which never 
liquefy the medium. They scarcely exceed a millet seed in size and resemble rather 
cbsely the colonies of zoo deic tuberculosis, save that the latter are much less firmly ad¬ 
herent to the gelatine. 
Agar is a rather poor medium of culture, whether it be plain or glycerinized. If the 
material used to inoculate is vigorous, abundant -transparent iridescent colonies appear 
on the surface of the agar, and gradually become opaque and bluish white. 
The germ of influenza grows in milk which is not coagulated even after several weeks. 
Agar of Wiirtz and rubin agar are not reddened. 
