786 
VERANUS A. MOORE. 
some difficulty. He found that this organism soon escaped 
from the tissues but that it “ places the animal organism in an 
extraordinary state of susceptibility with regard to extraneous 
bacteria notably streptococci.” On this ground he explains 
the almost constant presence of streptococci in the lesions of 
this disease. 
It has been my privilege to make a bacteriologic examina¬ 
tion of the diseased organs from 5 cases of Brustseuche. All of 
these were diagnosed, and, when not too far advanced, treated 
by Dr. Law. The horses were from different stables in Ithaca. 
Dr. Law made the post-mortem examination in four of the 
cases, carefully removed the organs and brought them to the 
laboratory. In one case I assisted at the post-mortem. 
In each of the five cases the lungs were more or less hepat- 
ized but the other organs were nearly normal in appearance. 
Several tubes of bouillon and agar were inoculated, and agar 
plates were made from each lung and in three cases from the 
liver, spleen, and kidneys. Without exception, a streptococcus 
appeared, usually in pure culture, from the lungs. The inocu¬ 
lated tubes from the other organs remained clear. The strepto¬ 
cocci isolated from the different cases were identical in their 
morphology and cultural manifestations and pathogenesis. 
A microscopic study of the lungs from the different horses 
showed micrococci single, in pairs and occasionally in short 
chains. Distinct capsules were not observed. In bouillon cult- 
Potato is not suitable for the cultivation of this microbe. 
Of all media the most favorable is peptonized bouillon to which a small quantity of 
serum has been added ” 
Search for the Microbe .—“ Inoculation and the direct cultivation of pathological 
products taken from horses affected with the disease as a rule do not give any results in -i 
ordinary conditions, but if one takes 4 or 5 c.c. of blood, pleuritic liquid, or pulmonary 
serosity, and injects the material into the peritoneum of a guinea pig, the animal not I 
rarely succumbs to a peritonitis, in the liquid of which the microbe of influenza is en- ' 
countered in considerable numbers. Nasal discharge, whether sanious or not, taken es¬ 
pecially at the beginning still more frequently gives a positive result. If the culture is 
not pure one inoculates in succession, into the peritoneum, one, two, or three guinea- 
pigs, and very soon only the bacillus of influenza shows itself. 
This microbe retains its virulence for a long time in peritoneal liquid, and when 
transferred thence to artificial media it grows in them abundantly.” 
