348 
J. D. SPRAGUE. 
another pen and the entire 98 head treated with serum None 
died until the third day after treatment, when those of the in¬ 
fected lot began dying, the disease taking its regular course. 
This continued for several days, when I gave the infected ones 
another injection of serum. None died until the third day after 
the second injection, when they again began dying. _ I then re¬ 
peated the inoculation, which seemed to check the disease again 
for three days, when they began dying and continued until the 
entire 45 with 2 of the 55 were dead. The 53 remained per¬ 
fectly healthy. About 10 days after the first treatment, one of 
the healthy animals which had become slightly crippled 111 some 
way was placed with the sick ones. This remained well, and 
after the sick ones had all died it was replaced with the • ongi- 
nal bunch. . . . 
Lot VI. Treated 60 head in feed lot containing about 150 
head. A few were dying with symptoms of cholera. Very few 
of the treated animals died, but owing to many of the original 
hogs being taken out and replaced by others the per cent, cou c 
not be obtained. . , 
Lot VII. 18 head that were left of a bunch of aDout 50, tiie 
others having died of cholera. These were each given one dose 
of serum. The second day about one-half of them were given a 
second dose ; the third day those presenting the worst symp¬ 
toms were again treated and on the fourth day the same. None 
died until about one week after treatment. At this time some 
of them showed symptoms of being worse and 4 died, 14 ma - 
ing a nice recovery. . . t 
You will notice that in some cases I have been repeating the 
doses. In the next experiment, Lot 8, I have taken extra care 
to ascertain if there is any benefit in/so doing ; selected subjects 
in which the symptoms were well marked from an mfecte 
herd. These were separated from the balance of the herd and 
treated once daily until showing improvement or dying. 
Treated 17 head, four of them dying. Four of the herd not 
treated also died. Some only required 2 doses, while one too.c 
ten. Two of the four which died died with symptoms of cholera. 
